ArticleScientific reports2016
Fast antibody fragment motion: flexible linkers act as entropic spring.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Four IgG Antibodies and Protein G Are Shapeshifters.International journal of molecular sciences · 2026Article
- Diffusion, search and attack motions of antibodies.Communications biology · 2025Article
- Dynamical Signatures ofACS omega · 2025Article
- Review
- Intact Transition Epitope Mapping-Force Interferences by Variable Extensions (ITEM-FIVE).Biomolecules · 2024Article
- Interchain Hydrodynamic Interaction and Internal Friction of Polyelectrolytes.ACS macro letters · 2023Article
- Molecular Flexibility of Antibodies Preserved Even in the Dense Phase after Macroscopic Phase Separation.Molecular pharmaceutics · 2021Article
- Article
- The SARS-CoV-2 Spike variant D614G favors an open conformational state.Science advances · 2021Article
- Intermediate scattering functions of a rigid body monoclonal antibody protein in solution studied by dissipative particle dynamic simulation.Structural dynamics (Melville, N.Y.) · 2021Article
- Transition between protein-like and polymer-like dynamic behavior: Internal friction in unfolded apomyoglobin depends on denaturing conditions.Scientific reports · 2020Article
- Atomistic Modeling of Scattering Curves for Human IgG1/4 Reveals New Structure-Function Insights.Biophysical journal · 2019Article
- Evaluating the Effects of Hinge Flexibility on the Solution Structure of Antibodies at Concentrated Conditions.Journal of pharmaceutical sciences · 2019Article
- The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes.Biophysical journal · 2018Article
- Rolling Adhesion of Schizont Stage Malaria-Infected Red Blood Cells in Shear Flow.Biophysical journal · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A flexible linker region between three fragments allows antibodies to adjust their binding sites to an antigen or receptor. Using Neutron Spin Echo Spectroscopy we observed fragment motion on a timescale of 7 ns with motional amplitudes of about 1 nm relative to each other. The mechanistic complexity of the linker region can be described by a spring model with Brownian motion of the fragments in a harmonic potential. Displacements, timescale, friction and force constant of the underlying dynamics are accessed. The force constant exhibits a similar strength to an entropic spring, with friction of the fragment matching the unbound state. The observed fast motions are fluctuations in pre-existing equilibrium configurations. The Brownian motion of domains in a harmonic potential is the appropriate model to examine functional hinge motions dependent on the structural topology and highlights the role of internal forces and friction to function.
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