ArticleScientific reports2016
Conductive resins improve charging and resolution of acquired images in electron microscopic volume imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 107 citations in OpenAlex.
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- Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers.PloS one · 2024Article
- Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest.Frontiers in zoology · 2023Article
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- Whole-cell observation of ZIO-stained Golgi apparatus in rat hepatocytes with serial block-face scanning electron microscope, SBF-SEM.Microscopy (Oxford, England) · 2022Article
- Region specificity of fibroblast-like cells in the mucosa of the rat large intestine.Cell and tissue research · 2022Article
- How innovations in methodology offer new prospects for volume electron microscopy.Journal of microscopy · 2022Review
- Landmark-based retrieval of inflamed skin vessels enabled by 3D correlative intravital light and volume electron microscopy.Histochemistry and cell biology · 2022Article
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Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in serial block-face imaging using scanning electron microscopy (SEM) have enabled the rapid and efficient acquisition of 3-dimensional (3D) ultrastructural information from a large volume of biological specimens including brain tissues. However, volume imaging under SEM is often hampered by sample charging, and typically requires specific sample preparation to reduce charging and increase image contrast. In the present study, we introduced carbon-based conductive resins for 3D analyses of subcellular ultrastructures, using serial block-face SEM (SBF-SEM) to image samples. Conductive resins were produced by adding the carbon black filler, Ketjen black, to resins commonly used for electron microscopic observations of biological specimens. Carbon black mostly localized around tissues and did not penetrate cells, whereas the conductive resins significantly reduced the charging of samples during SBF-SEM imaging. When serial images were acquired, embedding into the conductive resins improved the resolution of images by facilitating the successful cutting of samples in SBF-SEM. These results suggest that improving the conductivities of resins with a carbon black filler is a simple and useful option for reducing charging and enhancing the resolution of images obtained for volume imaging with SEM.
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