ArticlePlant methods2016
Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling.
Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in Arabidopsis Seedlings.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optimization of cassava (MethodsX · 2024Article
- Insights Into Plant Surgery: An Overview of the Multiple Grafting Techniques forFrontiers in plant science · 2020Review
- Shoot/Root Interactions Affect Soybean Photosynthetic Traits and Yield Formation: A Case Study of Grafting With Record-Yield Cultivars.Frontiers in plant science · 2019Article
- Bioinformatic and expression analysis of the Brassica napus L. cyclophilins.Scientific reports · 2017Article
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Authors and funding
5 authors.
Funding
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Abstract
backgroundGrafting is a well-established technique for studying long-distance transport and signalling processes in higher plants. While oilseed rape has been the subject of comprehensive analyses of xylem and phloem sap to identify macromolecules potentially involved in long-distance information transfer, there is currently no standardised grafting method for this species published.
resultsWe developed a straightforward collar-free grafting protocol for Brassica napus plants with high reproducibility and success rates. Micrografting of seedlings was done on filter paper. Grafting success on different types of regeneration media was measured short-term after grafting and as the long-term survival rate (>14 days) of grafts after the transfer to hydroponic culture or soil.
conclusionsWe compared different methods for grafting B. napus seedlings. Grafting on filter paper with removed cotyledons, a truncated hypocotyl and the addition of low levels of sucrose under long day conditions allowed the highest grafting success. A subsequent long-term hydroponic cultivation of merged grafts showed highest survival rates and best reproducibility.
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