Evidence map›Paper›PMID 27018858›Full record

ArticlePloS one2016

Comparative Genomic Analysis of Escherichia coli O157:H7 Isolated from Super-Shedder and Low-Shedder Cattle.

Krysty D Munns, Rahat Zaheer, Yong Xu, Kim Stanford, Chad R Laing, Victor P J Gannon, L Brent Selinger, Tim A McAllister

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Krysty D MunnsAgriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada.
Rahat ZaheerAgriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada.
Yong XuDepartment of Biological Sciences, University of Lethbridge, Lethbridge AB, Canada.
Kim StanfordAgriculture and Forestry, Lethbridge, AB, Canada.
Chad R LaingLaboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, AB, Canada.
Victor P J GannonLaboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, AB, Canada.
L Brent SelingerDepartment of Biological Sciences, University of Lethbridge, Lethbridge AB, Canada.
Tim A McAllisterAgriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada.
Agriculture and Agri-Food Canada · CAPublic Health Agency of Canada · CAUniversity of Lethbridge · CALethbridge Research and Development Centre

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cattle are the primary reservoir of the foodborne pathogen Escherichia coli O157:H7, with the concentration and frequency of E. coli O157:H7 shedding varying substantially among individual hosts. The term ''super-shedder" has been applied to cattle that shed ≥10(4) cfu E. coli O157:H7/g of feces. Super-shedders have been reported to be responsible for the majority of E. coli O157:H7 shed into the environment. The objective of this study was to determine if there are phenotypic and/or genotypic differences between E. coli O157:H7 isolates obtained from super-shedder compared to low-shedder cattle. From a total of 784 isolates, four were selected from low-shedder steers and six isolates from super-shedder steers (4.01-8.45 log cfu/g feces) for whole genome sequencing. Isolates were phage and clade typed, screened for substrate utilization, pH sensitivity, virulence gene profiles and Stx bacteriophage insertion (SBI) sites. A range of 89-2473 total single nucleotide polymorphisms (SNPs) were identified when sequenced strains were compared to E. coli O157:H7 strain Sakai. More non-synonymous SNP mutations were observed in low-shedder isolates. Pan-genomic and SNPs comparisons did not identify genetic segregation between super-shedder or low-shedder isolates. All super-shedder isolates and 3 of 4 of low-shedder isolates were typed as phage type 14a, SBI cluster 3 and SNP clade 2. Super-shedder isolates displayed increased utilization of galactitol, thymidine and 3-O-β-D-galactopyranosyl-D-arabinose when compared to low-shedder isolates, but no differences in SNPs were observed in genes encoding for proteins involved in the metabolism of these substrates. While genetic traits specific to super-shedder isolates were not identified in this study, differences in the level of gene expression or genes of unknown function may still contribute to some strains of E. coli O157:H7 reaching high densities within bovine feces.

Indexed as

Bacterial SheddingAnimalsBacterial Typing TechniquesCattleCattle DiseasesColony Count, MicrobialEscherichia coli InfectionsEscherichia coli O157FecesGenome, BacterialGenomicsHydrogen-Ion ConcentrationPhylogenyPolymorphism, Single NucleotideSequence Analysis, DNA

Identifiers

PMID27018858
PMCPMC4809568
OpenAlexW2322233872

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.