Modulating intracellular acidification by regulating the incubation time of proton caged compounds.

Marilena Carbone, Gianfranco Sabbatella, Simonetta Antonaroli, Viviana Orlando, Stefano Biagioni, Alessandro Nucara

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Article in European biophysics journal : EBJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Marilena CarboneDepartment of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy. marilena.carbone@roma2.infn.it.ORCID http://orcid.org/0000-0002-4224-1393

Gianfranco SabbatellaDepartment of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy.

Simonetta AntonaroliDepartment of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy.

Viviana OrlandoDepartment of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.

Stefano BiagioniDepartment of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.

Alessandro NucaraDepartment of Physics, University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A proton caged compound, the 1-(2-nitrophenyl)- ethylhexadecyl sulfonate (HDNS), was dosed into HEK-293 at different incubation times. Samples were irradiated with filtered UV light for inducing photolysis of the HDNS and then probed by infrared spectroscopy. The intracellular acidification reaction can be followed by monitoring the consequent CO2 peak intensity variation. The total CO2 produced is similar for all the samples, hence it is only a function of the initial HDNS concentration. The way it is achieved, though, is different for the different incubation times and follows kinetics, which results in a combination of a linear CO2 increase and a steep CO2 increase followed by a decay. This is interpreted in terms of confinement of the HDNS into intracellular vesicles of variable average size and sensitive to UV light when they reach critical dimensions.

Indexed as

ProtonsCarbon DioxideHEK293 CellsHumansHydrogen-Ion ConcentrationIntracellular SpaceSulfonic Acids1-(2-nitrophenyl)-ethylhexadecylsulfonateCarbon DioxideProtonsSulfonic AcidsCO2 monitoringIntracellular acidificationProton caged compounds

Identifiers

PMID27017356

DOI10.1007/s00249-016-1122-5

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