ArticleThe protein journal2016
Comparison of Different Protein Extraction Methods for Gel-Based Proteomic Analysis of Ganoderma spp.
Article in The protein journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Proteomic insights into fruit-pathogen interactions: managing biotic stress in fruit.Plant cell reports · 2025Review
- Recovery of Proteases and Protease Inhibitors fromCurrent protein & peptide science · 2025Article
- Traditional to technological advancements in Ganoderma detection methods in oil palm.Folia microbiologica · 2024Review
- An In-Depth Study of PhytopathogenicJournal of fungi (Basel, Switzerland) · 2024Review
- Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus.Biotechnology letters · 2022Article
- Differential Analysis of Mycelial Proteins and Metabolites From Rigidoporus Microporus During In Vitro Interaction With Hevea Brasiliensis.Microbial ecology · 2022Article
- Growth performance ofSaudi journal of biological sciences · 2021Article
- Optimization of Protein Extraction Method for 2DE Proteomics of Goat's Milk.Molecules (Basel, Switzerland) · 2020Article
- Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate inInternational journal of molecular sciences · 2019Article
- An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS.BMC genomics · 2019Article
- Application of Proteomics Technologies in Oil Palm Research.The protein journal · 2018Review
- Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ganoderma species are a group of fungi that have the ability to degrade lignin polymers and cause severe diseases such as stem and root rot and can infect economically important plants and perennial crops such as oil palm, especially in tropical countries such as Malaysia. Unfortunately, very little is known about the complex interplay between oil palm and Ganoderma in the pathogenesis of the diseases. Proteomic technologies are simple yet powerful tools in comparing protein profile and have been widely used to study plant-fungus interaction. A critical step to perform a good proteome research is to establish a method that gives the best quality and a wide coverage of total proteins. Despite the availability of various protein extraction protocols from pathogenic fungi in the literature, no single extraction method was found suitable for all types of pathogenic fungi. To develop an optimized protein extraction protocol for 2-DE gel analysis of Ganoderma spp., three previously reported protein extraction protocols were compared: trichloroacetic acid, sucrose and phenol/ammonium acetate in methanol. The third method was found to give the most reproducible gels and highest protein concentration. Using the later method, a total of 10 protein spots (5 from each species) were successfully identified. Hence, the results from this study propose phenol/ammonium acetate in methanol as the most effective protein extraction method for 2-DE proteomic studies of Ganoderma spp.
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