ArticlePlant reproduction2016
The long pollen tube journey and in vitro pollen germination of Phalaenopsis orchids.
Article in Plant reproduction, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Optimizing gamma irradiation for genotype-specific enhancement of pollen performance and haploid induction in cucumber (Cucumis sativus L.).Scientific reports · 2026Article
- Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber.Scientific reports · 2024Article
- Effects of Pollen Sources on Fruit Set and Fruit Characteristics of 'Fengtangli' Plum (International journal of molecular sciences · 2022Article
- Co-option of the SHOOT MERISTEMLESS network regulates protocorm-like body development in Phalaenopsis aphrodite.Plant physiology · 2022Article
- First Record of Ategmic Ovules in Orchidaceae Offers New Insights Into Mycoheterotrophic Plants.Frontiers in plant science · 2019Article
- Morphological and Molecular Characterization of Orchid Fruit Development.Frontiers in plant science · 2019Article
- Pollen Developmental Arrest: Maintaining Pollen Fertility in a World With a Changing Climate.Frontiers in plant science · 2019Review
- Article
- A Protoplast Transient Expression System to Enable Molecular, Cellular, and Functional Studies inFrontiers in plant science · 2018Article
- Cell-cell communications and molecular mechanisms in plant sexual reproduction.Journal of plant research · 2018Review
- Expression analysis of fertilization/early embryogenesis-associated genes in Phalaenopsis orchids.Plant signaling & behavior · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messagePollen biology in P. aphrodite. Orchids have a distinct reproductive program. Pollination triggers ovule development and differentiation within flowers, and fertilization occurs days to months after pollination. It is unclear how pollen tubes travel through the developing ovaries during ovule development and when pollen tubes arrive at the mature embryo sac to achieve fertilization. Here, we report a robust staining protocol to image and record the timing of pollen germination, progressive growth of pollen tubes in ovaries, and arrival of pollen tubes at embryo sacs in Phalaenopsis aphrodite. The pollen germinated and pollen tubes entered the ovary 3 days after pollination. Pollen tubes continued to grow and filled the entire cavity of the ovary as the ovary elongated and ovules developed. Pollen tubes were found to enter the matured embryo sacs at approximately 60-65 days after pollination in an acropetal manner. Moreover, these temporal changes in developmental events such as growth of pollen tubes and fertilization were associated with expression of molecular markers. In addition, we developed an in vitro pollen germination protocol, which is valuable to enable studies on pollen tube guidance and tip growth regulation in Phalaenopsis orchids and possibly in other orchid species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.