ArticleJournal of structural biology2016
Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography.
Article in Journal of structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET.Research square · 2025Article
- TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET.bioRxiv : the preprint server for biology · 2024Article
- Missing Wedge Completion via Unsupervised Learning with Coordinate Networks.International journal of molecular sciences · 2024Article
- Missing Wedge Completion via Unsupervised Learning with Coordinate Networks.bioRxiv : the preprint server for biology · 2024Article
- The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography.Frontiers in molecular biosciences · 2024Review
- Previously uncharacterized rectangular bacterial structures in the dolphin mouth.Nature communications · 2023Article
- Computational methods forFrontiers in cellular and infection microbiology · 2023Review
- Article
- Efficient manual annotation of cryogenic electron tomograms using IMOD.STAR protocols · 2022Article
- A feature-guided, focused 3D signal permutation method for subtomogram averaging.Journal of structural biology · 2022Article
- High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.Nature protocols · 2022Review
- Conformational Changes of RORγ During Response Element Recognition and Coregulator Engagement.Journal of molecular biology · 2021Article
- Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates.Communications biology · 2021Article
- Tools for visualizing and analyzing Fourier space sampling in Cryo-EM.Progress in biophysics and molecular biology · 2021Review
- A guided approach for subtomogram averaging of challenging macromolecular assemblies.Journal of structural biology: X · 2020Article
- Advances in cryo-electron tomography and subtomogram averaging and classification.Current opinion in structural biology · 2019Review
- Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes.Acta crystallographica. Section D, Structural biology · 2019Article
- Neutralizing Antibodies Inhibit Chikungunya Virus Budding at the Plasma Membrane.Cell host & microbe · 2018Article
- Fine details in complex environments: the power of cryo-electron tomography.Biochemical Society transactions · 2018Review
- Flagellum couples cell shape to motility inProceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Single particle cryo-electron tomography (cryoSPT) extracts features from cryo-electron tomograms, followed by 3D classification, alignment and averaging to generate improved 3D density maps of such features. Robust methods to correct for the contrast transfer function (CTF) of the electron microscope are necessary for cryoSPT to reach its resolution potential. Many factors can make CTF correction for cryoSPT challenging, such as lack of eucentricity of the specimen stage, inherent low dose per image, specimen charging, beam-induced specimen motions, and defocus gradients resulting both from specimen tilting and from unpredictable ice thickness variations. Current CTF correction methods for cryoET make at least one of the following assumptions: that the defocus at the center of the image is the same across the images of a tiltseries, that the particles all lie at the same Z-height in the embedding ice, and/or that the specimen, the cryo-electron microscopy (cryoEM) grid and/or the carbon support are flat. These experimental conditions are not always met. We have developed a CTF correction algorithm for cryoSPT without making any of the aforementioned assumptions. We also introduce speed and accuracy improvements and a higher degree of automation to the subtomogram averaging algorithms available in EMAN2. Using motion-corrected images of isolated virus particles as a benchmark specimen, recorded with a DE20 direct detection camera, we show that our CTF correction and subtomogram alignment routines can yield subtomogram averages close to 4/5 Nyquist frequency of the detector under our experimental conditions.
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