ArticleCell2016
Heterogeneity in Oct4 and Sox2 Targets Biases Cell Fate in 4-Cell Mouse Embryos.
Article in Cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 221 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
221 citing papers in PubMed, 515 citations in OpenAlex.
- Interpretable scRNA-seq Analysis with Intelligent Gene Selection.Applied biochemistry and biotechnology · 2026Article
- Two distributed enhancers of Sox17 additively regulate the developmental fate of bipotential ventral pancreato-biliary progenitor cells.Development (Cambridge, England) · 2026Article
- Phase Separation of SF3B1 Serves as a Critical Post-Transcriptional Regulator During Early Mouse Embryogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-cell marker gene clustering: A unified deep learning framework for marker gene-based clustering of single-cell RNA-sequencing data.Quantitative biology (Beijing, China) · 2026Article
- Asymmetric RBBP7 regulates the first cell fate decision of early mammalian embryos.Cell discovery · 2026Article
- Subcellularly Resolved 3D Translatome in Mouse Oocytes and Early Embryos.bioRxiv : the preprint server for biology · 2026Article
- Early transcriptional divergence underlies cell fate bias in bovine embryos.Communications biology · 2026Article
- scMVAF: a multi-view adaptive fusion clustering approach for single-cell RNA-sequencing data.Briefings in bioinformatics · 2026Article
- High-sensitivity in situ detection of Sox21 reveals transcript asymmetry from the zygote stage.Developmental biology · 2026Article
- Beyond the mean: genetic control of gene expression fidelity and dispersion.bioRxiv : the preprint server for biology · 2026Article
- Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development.Protein & cell · 2026Article
- scDBic: a novel deep learning-based biclustering algorithm for analyzing scRNA-seq data.Bioinformatics (Oxford, England) · 2026Article
- Distinct roles for SETα and SETβ in early cell fate decisions.Nucleic acids research · 2026Article
- Imputing missing values in single-cell RNA-sequencing data: a statistical and machine learning-based approach.Briefings in bioinformatics · 2026Article
- What makes genes burst.Trends in cell biology · 2026Review
- Article
- Crosstalk between H3K4me3 and oxidative stress is a potential target for the improvement of ART-derived embryos.Nature communications · 2025Article
- Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes.Nature communications · 2025Article
- Analytic Fourier ptychotomography for aberration-free and high-resolution volumetric refractive index imaging.Nature communications · 2025Article
- Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification.eLife · 2025Article
161 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The major and essential objective of pre-implantation development is to establish embryonic and extra-embryonic cell fates. To address when and how this fundamental process is initiated in mammals, we characterize transcriptomes of all individual cells throughout mouse pre-implantation development. This identifies targets of master pluripotency regulators Oct4 and Sox2 as being highly heterogeneously expressed between blastomeres of the 4-cell embryo, with Sox21 showing one of the most heterogeneous expression profiles. Live-cell tracking demonstrates that cells with decreased Sox21 yield more extra-embryonic than pluripotent progeny. Consistently, decreasing Sox21 results in premature upregulation of the differentiation regulator Cdx2, suggesting that Sox21 helps safeguard pluripotency. Furthermore, Sox21 is elevated following increased expression of the histone H3R26-methylase CARM1 and is lowered following CARM1 inhibition, indicating the importance of epigenetic regulation. Therefore, our results indicate that heterogeneous gene expression, as early as the 4-cell stage, initiates cell-fate decisions by modulating the balance of pluripotency and differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.