ArticleFrontiers in microbiology2016
Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn).
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 103 citations in OpenAlex.
- A RUBY-Based Visual Hairy Root Transformation and CRISPR-Plants (Basel, Switzerland) · 2026Article
- Specific features of rol-gene polymorphism of Rhizobium rhizogenes.Archives of microbiology · 2026Review
- Establishment of a TRV-based virus-induced gene silencing system using the vacuum infiltration in Tartary buckwheat.Plant cell reports · 2025Article
- A robust in-vitro and ex-vitro Agrobacterium rhizogenes-mediated hairy root transformation system in mungbean for efficient visual screening of transformants using the RUBY reporter.BMC plant biology · 2025Article
- EfficientInternational journal of molecular sciences · 2025Article
- Compounds from medicinal plants produced in hairy root and transgenic hairy root cultures: a review.PeerJ · 2025Review
- Article
- Accumulation of phenolic compounds and expression of phenylpropanoid biosynthesis-related genes in leaves of basil transformed withPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Efficient Agrobacterium-mediated transformation and genome editing ofFrontiers in plant science · 2023Article
- Optimization ofFrontiers in plant science · 2023Article
- Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins.Plants (Basel, Switzerland) · 2022Review
- Buckwheat in Tissue Culture Research: Current Status and Future Perspectives.International journal of molecular sciences · 2022Review
- Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis.BMC plant biology · 2022Article
- Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum.Frontiers in plant science · 2022Article
- Generation of Stable Catharanthus roseus Hairy Root Lines with Agrobacterium rhizogenes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space.Frontiers in plant science · 2022Article
- Using machine learning to link the influence of transferredFrontiers in plant science · 2022Article
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
- A Brief Overview of Potential Treatments for Viral Diseases Using Natural Plant Compounds: The Case of SARS-Cov.Molecules (Basel, Switzerland) · 2021Review
- Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots OverexpressingInternational journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of an efficient protocol for successful hairy root induction by Agrobacterium rhizogenes is the key step toward an in vitro culturing method for the mass production of secondary metabolites. The selection of an effective Agrobacterium strain for the production of hairy roots is highly plant species dependent and must be determined empirically. Therefore, our goal was to investigate the transformation efficiency of different A. rhizogenes strains for the induction of transgenic hairy roots in Fagopyrum tataricum 'Hokkai T10' cultivar; to determine the expression levels of the polypropanoid biosynthetic pathway genes, such as ftpAL, FtC4H, Ft4CL, FrCHS, FrCH1, FrF3H, FtFLS1, FtFLS2, FtF3(,) H1, FtF3'H2, FtANS, and FtDFR; and to quantify the in vitro synthesis of phenolic compounds and anthocyanins. Among different strains, R1000 was the most promising candidate for hairy root stimulation because it induced the highest growth rate, root number, root length, transformation efficiency, and total anthocyanin and rutin content. The R1000, 15834, and A4 strains provided higher transcript levels for most metabolic pathway genes for the synthesis of rutin (22.31, 15.48, and 13.04 μg/mg DW, respectively), cyanidin 3-O-glucoside (800, 750, and 650 μg/g DW, respectively), and cyanidin 3-O-rutinoside (2410, 1530, and 1170 μg/g DW, respectively). A suitable A. rhizogenes strain could play a vital role in the fast growth of the bulk amount of hairy roots and secondary metabolites. Overall, R1000 was the most promising strain for hairy root induction in buckwheat.
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