Evidence map›Paper›PMID 27011896›Full record

ArticleApplications in plant sciences2016

Identification and characterization of microsatellite markers in Penstemon scariosus (Plantaginaceae).

Chris D Anderson, Nathan J Ricks, Kevin M Farley, Peter J Maughan, Mikel R Stevens

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Article in Applications in plant sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Chris D AndersonDepartment of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
Nathan J RicksDepartment of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
Kevin M FarleyDepartment of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
Peter J MaughanDepartment of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.
Mikel R StevensDepartment of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602 USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyPenstemon scariosus var. albifluvis (Plantaginaceae) has been proposed to be federally listed as threatened due to its unique, geologically oil-rich habitat. Developing simple sequence repeat (SSR) markers to study its genetic diversity would be most useful. METHODS AND

resultsUsing genomic reduction in combination with next-generation sequencing, we identified SSR motifs with five to 15 perfect repeats in 1067 P. scariosus contigs. After multiple qualifying tests, 16 SSRs were selected for their robust polymorphic reliability across 12 taxa with as high as 21 alleles in a given taxon. With the exception of two monomorphic loci, the observed and expected heterozygosity values ranged from 0.083 to 1.000 and 0.398 to 0.920, respectively.

conclusionsThese microsatellite markers will directly aid in studies of the genetic diversity and relatedness of P. scariosus, P. comarrhenus, P. compactus, P. cyananthus var. cyananthus, P. fremontii var. fremontii, P. fremontii var. glabrescens, P. gibbensii, P. strictus, and P. subglaber.

Indexed as

454 sequencingcross-amplificationPenstemon scariosus var. albifluvisPlantaginaceaesimple sequence repeat (SSR) markersWhite River penstemon

Identifiers

PMID27011896
PMCPMC4795917

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