ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2016
Aerobic culture of methanogenic archaea without an external source of hydrogen.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- Tobacco-smoking-related prevalence of methanogens in the oral fluid microbiota.Scientific reports · 2018Trial
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- The oral microbiome: diversity, biogeography and human health.Nature reviews. Microbiology · 2024Review
- Article
- Human associated Archaea: a neglected microbiome worth investigating.World journal of microbiology & biotechnology · 2024Review
- Review
- Review
- Crosstalk between the Resident Microbiota and the Immune Cells Regulates Female Genital Tract Health.Life (Basel, Switzerland) · 2023Review
- Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.Microorganisms · 2023Review
- Trait biases in microbial reference genomes.Scientific data · 2023Article
- Functional biomes beyond the bacteriome in the oral ecosystem.The Japanese dental science review · 2022Review
- Rapid identification of clinically interesting methanogens using an improved MALDI-TOF-MS assay.Access microbiology · 2022Article
- Scope of Archaea in Fish Feed: a New Chapter in Aquafeed Probiotics?Probiotics and antimicrobial proteins · 2021Review
- State of the Art in the Culture of the Human Microbiota: New Interests and Strategies.Clinical microbiology reviews · 2020Review
- Detection ofMicroorganisms · 2020Article
- Article
- The Antioxidants Glutathione, Ascorbic Acid and Uric Acid Maintain Butyrate Production by Human Gut Clostridia in The Presence of Oxygen In Vitro.Scientific reports · 2020Article
- Syntrophy via Interspecies HmBio · 2020Article
- Culture of Methanogenic Archaea from Human Colostrum and Milk.Scientific reports · 2019Article
- First characterization of methanogens in oral cavity in Malian patients with oral cavity pathologies.BMC oral health · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culturing methanogenic archaea is fastidious, expensive, and requires an external source of hydrogen and carbon dioxide. Until now, these microorganisms have only been cultivated under strictly anaerobic conditions. We previously developed a single versatile culture medium containing sugars and anti-oxydants for cultivating all human known methanogens. Performing aerobic cultures in the presence of Bacteroides thetaiotaomicron, which produces hydrogen, allows for cultivation of Methanobrevibacter smithii which itself produces methane. To obtain colonies, we cultivated M. smithii in an agar plate in the upper part of a double chamber flask with a liquid culture of B. thetaiotaomicron in the lower compartment. We subsequently cultured four other methanogenic species for the first time and successfully isolated 13 strains of M. smithii and nine strains of Methanobrevibacter oralis from 100 stools and 45 oral samples. This procedure allows aerobic isolation and antibiotic susceptibility testing. This changes the ability to routinely study methanogens, which have been neglected in clinical microbiology laboratories and may be useful for biogas production.
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Registered trials
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