ArticleBiochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology2016
An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes.
Article in Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Biochemistry Learning in Higher Education: A Systematic Review on Methodologies and Teaching Resources.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular BiologyPooled it
- A genetic screen of transcription factors in the Drosophila melanogaster abdomen identifies novel pigmentation genes.G3 (Bethesda, Md.) · 2024Article
- "Designer babies?!" A CRISPR-based learning module for undergraduates built around the CCR5 gene.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2021Article
- Integrating CRISPR-Cas9 Technology into Undergraduate Courses: Perspectives from a National Science Foundation (NSF) Workshop for Undergraduate Faculty, June 2018.Journal of microbiology & biology education · 2019Article
- An Undergraduate Research Project Utilizing CRISPR-Cas9 Gene Editing Technology to Study Gene Function inJournal of microbiology & biology education · 2019Article
- CRISPR Gene Editing in Yeast: An Experimental Protocol for an Upper-Division Undergraduate Laboratory Course.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2018Article
- Absence of the Drosophila jump muscle actin Act79B is compensated by up-regulation of Act88F.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- CRISPR-Cas Technology In and Out of the Classroom.The CRISPR journal · 2018Article
- Functional redundancy and nonredundancy between two Troponin C isoforms inMolecular biology of the cell · 2017Article
- An undergraduate research experience in CRISPR-Cas9 mediated eukaryotic genome editing to teach fundamental biochemistry techniques.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular BiologyArticle
- An effective Caenorhabditis elegans CRISPR training module for high school and undergraduate summer research experiences in molecular biology.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular BiologyArticle
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
CRISPR/Cas9 genome editing technology is used in the manipulation of genome sequences and gene expression. Because of the ease and rapidity with which genes can be mutated using CRISPR/Cas9, we sought to determine if a single-semester undergraduate class could be successfully taught, wherein students isolate mutants for specific genes using CRISPR/Cas9. Six students were each assigned a single Drosophila gene, for which no mutants currently exist. Each student designed and created plasmids to encode single guide RNAs that target their selected gene; injected the plasmids into Cas9-expressing embryos, in order to delete the selected gene; carried out a three-generation cross to test for germline transmission of a mutated allele and generate a stable stock of the mutant; and characterized the mutant alleles by PCR and sequencing. Three genes out of six were successfully mutated. Pre- and post- survey evaluations of the students in the class revealed that student attitudes towards their research competencies increased, although the changes were not statistically significant. We conclude that it is feasible to develop a laboratory genome editing class, to provide effective laboratory training to undergraduate students, and to generate mutant lines for use by the broader scientific community. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:263-275, 2016.
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