Evidence map›Paper›PMID 27009592›Full record

ArticleFolia microbiologica2016

Comparative analysis of quantitative methodologies for Vibrionaceae biofilms.

Alba A Chavez-Dozal, Neda Nourabadi, Martina Erken, Diane McDougald, Michele K Nishiguchi

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Folia microbiologica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alba A Chavez-DozalSection of Infectious Diseases, New Mexico Veterans Healthcare System, Albuquerque, NM, 87108, USA.
Neda NourabadiDepartment of Biology, New Mexico State University, Box 30001, MSC 3AF, Las Cruces, NM, 88003-8001, USA.
Martina ErkenCentre for Marine Biofouling and Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of South Wales, Sydney, NSW, 2052, Australia.
Diane McDougaldithree Institute, Faculty of Science, University of Technology, Sydney, Broadway, Sydney, NSW, 2007, Australia.
Michele K NishiguchiDepartment of Biology, New Mexico State University, Box 30001, MSC 3AF, Las Cruces, NM, 88003-8001, USA. nish@nmsu.edu.ORCID http://orcid.org/0000-0002-8950-4621

Funding

RISE PROGRAM AT NEW MEXICO STATE UNIVERSITYR25GM061222 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI HOUSTON, JESSICA PEREA, REDDI, LAKSHMI · 2000 to 2022
$21.3M
FAIR Data Competency and Machine Learning Readiness for Biomedical ScientistsK12GM088021 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI REBECCA S. HARTLEY, Diane Lidke · 2009 to 2026
$16.4M
Biology of Infectious Diseases &InflammationT32AI007538 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Michelle A Ozbun · 1998 to 2026
$5.2M
NIAID NIH HHS T32 AI007538NIGMS NIH HHS K12 GM088021NIGMS NIH HHS R25 GM061222
6 · The paper itself

Abstract

Multiple symbiotic and free-living Vibrio spp. grow as a form of microbial community known as a biofilm. In the laboratory, methods to quantify Vibrio biofilm mass include crystal violet staining, direct colony-forming unit (CFU) counting, dry biofilm cell mass measurement, and observation of development of wrinkled colonies. Another approach for bacterial biofilms also involves the use of tetrazolium (XTT) assays (used widely in studies of fungi) that are an appropriate measure of metabolic activity and vitality of cells within the biofilm matrix. This study systematically tested five techniques, among which the XTT assay and wrinkled colony measurement provided the most reproducible, accurate, and efficient methods for the quantitative estimation of Vibrionaceae biofilms.

Indexed as

Bacteriological TechniquesBiofilmsVibrionaceae

Identifiers

PMID27009592
PMCPMC5035616

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.