Evidence map›Paper›PMID 27009517›Full record

ArticleLab on a chip2016

Mass spectrometry-based monitoring of millisecond protein-ligand binding dynamics using an automated microfluidic platform.

Yongzheng Cong, Shanta Katipamula, Cameron D Trader, Daniel J Orton, Tao Geng, Erin S Baker, Ryan T Kelly

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Quantum Dot Labelling of Tepary Bean (Molecules (Basel, Switzerland) · 2020
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yongzheng CongEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
Shanta KatipamulaEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
Cameron D TraderEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
Daniel J OrtonBiological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
Tao GengEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.
Erin S BakerBiological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
Ryan T KellyEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. ryan.kelly@pnnl.gov.

Funding

Platform Providing Increased Throughput, Sensitivity and Specificity for MetaboloR01ES022190 · NIEHS · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI BAKER, ERIN S · 2012 to 2016
$2.2M
High-throughput multidimensional bioseparations for next-generation proteomicsR21EB020976 · NIBIB · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI KELLY, RYAN T · 2016 to 2017
$400k
NIBIB NIH HHS R21 EB020976NIEHS NIH HHS R01 ES022190
6 · The paper itself

Abstract

Characterizing protein-ligand binding dynamics is crucial for understanding protein function and for developing new therapeutic agents. We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein-ligand binding dynamics. This platform offers many advantages, including solution-based binding, label-free detection, automated operation, rapid mixing, and low sample consumption.

Indexed as

Lab-On-A-Chip DevicesAlgorithmsAutomation, LaboratoryCarbonic Anhydrase ICarbonic Anhydrase InhibitorsDiffusionDiureticsEquipment DesignFurosemideHumansKineticsLigandsReproducibility of ResultsSpectrometry, Mass, Electrospray IonizationCarbonic Anhydrase ICarbonic Anhydrase InhibitorsDiureticsFurosemideLigands

Identifiers

PMID27009517
PMCPMC4846533

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.