ArticleAmerican journal of physiology. Renal physiology2016
Macrophage-derived IL-6 contributes to ANG II-mediated angiotensinogen stimulation in renal proximal tubular cells.
Article in American journal of physiology. Renal physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- The Mechano-Immune-Vascular Activation Loop: An Integrative Conceptual Framework for Positive Feedback in Hypertension.International journal of molecular sciences · 2026Review
- Validity of mental and physical stress models.Neuroscience and biobehavioral reviews · 2024Review
- IL-6 and diabetic kidney disease.Frontiers in immunology · 2024Review
- Glutamine Protects against Mouse Abdominal Aortic Aneurysm through Modulating VSMC Apoptosis and M1 Macrophage Activation.International journal of medical sciences · 2024Article
- STAT-3 signaling role in an experimental model of nephropathy induced by doxorubicin.Molecular and cellular biochemistry · 2023Article
- Immunosuppression by Mycophenolate Mofetil Mitigates Intrarenal Angiotensinogen Augmentation in Angiotensin II-Dependent Hypertension.International journal of molecular sciences · 2022Article
- The Role of Cytokines in Nephrotic Syndrome.Mediators of inflammation · 2022Review
- Angiotensin II biphasically regulates cell differentiation in human iPSC-derived kidney organoids.American journal of physiology. Renal physiology · 2021Article
- Berries and Their Polyphenols as a Potential Therapy for Coronary Microvascular Dysfunction: A Mini-Review.International journal of molecular sciences · 2021Review
- Amplification of Salt-Sensitive Hypertension and Kidney Damage by Immune Mechanisms.American journal of hypertension · 2021Review
- Aging- and gender-related modulation of RAAS: potential implications in COVID-19 disease.Vascular biology (Bristol, England) · 2021Review
- Kidney-intrinsic factors determine the severity of ischemia/reperfusion injury in a mouse model of delayed graft function.Kidney international · 2020Article
- Interferon Regulatory Factor-5 in Resident Macrophage Promotes Polycystic Kidney Disease.Kidney360 · 2020Article
- Blockade of sodium-glucose cotransporter 2 suppresses high glucose-induced angiotensinogen augmentation in renal proximal tubular cells.American journal of physiology. Renal physiology · 2020Article
- Sex-Specific Mechanisms in Inflammation and Hypertension.Current hypertension reports · 2019Review
- Immune mechanisms of salt-sensitive hypertension and renal end-organ damage.Nature reviews. Nephrology · 2019Review
- Advanced Glycation End Products Stimulate Angiotensinogen Production in Renal Proximal Tubular Cells.The American journal of the medical sciences · 2019Article
- Aliskiren Attenuates the Inflammatory Response and Wound Healing Process in Diabetic Mice With Periodontal Disease.Frontiers in pharmacology · 2019Article
- Canagliflozin Prevents Intrarenal Angiotensinogen Augmentation and Mitigates Kidney Injury and Hypertension in Mouse Model of Type 2 Diabetes Mellitus.American journal of nephrology · 2019Article
- Inflammation as a Regulator of the Renin-Angiotensin System and Blood Pressure.Current hypertension reports · 2018Review
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4 authors at 1 institution in 1 country.
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Abstract
The development of ANG II-dependent hypertension involves increased infiltration of macrophages (MΦ) and T cells into the kidney and the consequent elevation of intrarenal cytokines including IL-6, which facilitates the progression of hypertension and associated kidney injury. Intrarenal renin-angiotensin system (RAS) activation, including proximal tubular angiotensinogen (AGT) stimulation, has also been regarded as a cardinal mechanism contributing to these diseases. However, the interaction between immune cells and intrarenal RAS activation has not been fully delineated. Therefore, the present study investigated whether ANG II-treated MΦ induce AGT upregulation in renal proximal tubular cells (PTCs). MΦ were treated with 0-10(-6) M ANG II for up to 48 h. PTCs were incubated with the collected medium from MΦ. In ANG II-treated MΦ, IL-6 mRNA and protein levels were increased (1.86 ± 0.14, protein level, ratio to control); moreover, IL-6 levels were higher than TNF-α and IL-1β in culture medium isolated from ANG II-treated MΦ. Elevated AGT expression (1.69 ± 0.04, ratio to control) accompanied by phosphorylated STAT3 were observed in PTCs that received culture medium from ANG II-treated MΦ. The addition of a neutralizing IL-6 antibody to the collected medium attenuated phosphorylation of STAT3 and AGT augmentation in PTCs. Furthermore, a JAK2 inhibitor also suppressed STAT3 phosphorylation and AGT augmentation in PTCs. These results demonstrate that ANG II-induced IL-6 elevation in MΦ enhances activation of the JAK-STAT pathway and consequent AGT upregulation in PTCs, suggesting involvement of an immune response in driving intrarenal RAS activity.
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