Evidence map›Paper›PMID 27009329›Full record

ArticleScientific reports2016

Residual matrix from different separation techniques impacts exosome biological activity.

Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta, Paolo Bergese

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
101citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

101 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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  18. Isolation of extracellular vesicles from insect hemolymph.Current research in insect science · 2025
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41 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lucia PaoliniDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Andrea ZendriniDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Giuseppe Di NotoDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Sara BusattoDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Elisabetta LottiniDepartment of Chemistry and INSTM, Laboratory of Molecular Magnetism, University of Firenze, Sesto Fiorentino (Firenze), Italy.
Annalisa RadeghieriDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Alessandra DossiDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Andrea CaneschiDepartment of Chemistry and INSTM, Laboratory of Molecular Magnetism, University of Firenze, Sesto Fiorentino (Firenze), Italy.
Doris RicottaDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.
Paolo BergeseDepartment of Molecular and Translational Medicine and INSTM, University of Brescia, Brescia, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are gaining a prominent role in research due to their intriguing biology and several therapeutic opportunities. However, their accurate purification from body fluids and detailed physicochemical characterization remain open issues. We isolated exosomes from serum of patients with Multiple Myeloma by four of the most popular purification methods and assessed the presence of residual contaminants in the preparations through an ad hoc combination of biochemical and biophysical techniques - including Western Blot, colloidal nanoplasmonics, atomic force microscopy (AFM) and scanning helium ion microscopy (HIM). The preparations obtained by iodixanol and sucrose gradients were highly pure. To the contrary, those achieved with limited processing (serial centrifugation or one step precipitation kit) resulted contaminated by a residual matrix, embedding the exosomes. The contaminated preparations showed lower ability to induce NfkB nuclear translocation in endothelial cells with respect to the pure ones, probably because the matrix prevents the interaction and fusion of the exosomes with the cell membrane. These findings suggest that exosome preparation purity must be carefully assessed since it may interfere with exosome biological activity. Contaminants can be reliably probed only by an integrated characterization approach aimed at both the molecular and the colloidal length scales.

Indexed as

Cell FractionationCell NucleusCentrifugation, Density GradientExosomesHumansMicroscopy, Atomic ForceMultiple MyelomaNF-kappa BProtein TransportSucroseTriiodobenzoic AcidsiodixanolNF-kappa BSucroseTriiodobenzoic Acids

Identifiers

PMID27009329
PMCPMC4806376

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.