ArticleBMC genomics2016
Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Clonal dynamics deviate from neutral drift in zebrafish spermatogenesis.bioRxiv : the preprint server for biology · 2026Article
- Broad Vitamin BInternational journal of molecular sciences · 2025Article
- Transcriptional profiling of zebrafish identifies host factors controlling susceptibility to Shigella flexneri.Disease models & mechanisms · 2024Article
- Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish.Journal of visualized experiments : JoVE · 2023Article
- Review
- Evaluation of CRISPR gene-editing tools in zebrafish.BMC genomics · 2022Article
- High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing.Methods (San Diego, Calif.) · 2021Article
- The gene regulatory basis of genetic compensation during neural crest induction.PLoS genetics · 2019Article
- CRISPR/Cas9 Genome Editing Introduction and Optimization in the Non-model InsectFrontiers in physiology · 2019Article
- Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum).Nature protocols · 2018Article
- Article
- Multiplexed CRISPR/Cas9 Targeting of Genes Implicated in Retinal Regeneration and Degeneration.Frontiers in cell and developmental biology · 2018Article
- Generation of gene edited birds in one generation using sperm transfection assisted gene editing (STAGE).Transgenic research · 2017Article
- Loss of the homologous recombination geneProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.Nature reviews. Genetics · 2017Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe CRISPR/Cas9 system is a prokaryotic immune system that infers resistance to foreign genetic material and is a sort of 'adaptive immunity'. It has been adapted to enable high throughput genome editing and has revolutionised the generation of targeted mutations.
resultsWe have developed a scalable analysis pipeline to identify CRISPR/Cas9 induced mutations in hundreds of samples using next generation sequencing (NGS) of amplicons. We have used this system to investigate the best way to screen mosaic Zebrafish founder individuals for germline transmission of induced mutations. Screening sperm samples from potential founders provides much better information on germline transmission rates and crucially the sequence of the particular insertions/deletions (indels) that will be transmitted. This enables us to combine screening with archiving to create a library of cryopreserved samples carrying known mutations. It also allows us to design efficient genotyping assays, making identifying F1 carriers straightforward.
conclusionsThe methods described will streamline the production of large numbers of knockout alleles in selected genes for phenotypic analysis, complementing existing efforts using random mutagenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.