Evidence map›Paper›PMID 27009085›Full record

ArticleAnnals of biomedical engineering2016

Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors.

Sahar Ansari, Chider Chen, Xingtian Xu, Nasim Annabi, Homayoun H Zadeh, Benjamin M Wu, Ali Khademhosseini, Songtao Shi, Alireza Moshaverinia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Tissue Engineered 3D Constructs for Volumetric Muscle Loss.Annals of biomedical engineering · 2024
    Review
  9. Article
  10. Nanozyme-Based Robotics Approach for Targeting Fungal Infection.Advanced materials (Deerfield Beach, Fla.) · 2024
    Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Review
  16. Review
  17. Review
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sahar AnsariDivision of Growth and Development, School of Dentistry, University of California, Los Angeles, CA, USA.
Chider ChenSchool of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Xingtian XuOstrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.
Nasim AnnabiDepartment of Chemical Engineering, Northeastern University, Boston, MA, USA.
Homayoun H ZadehOstrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.
Benjamin M WuWeintraub Center for Reconstructive Biotechnology, Division of Advanced Prothodontics, School of Dentistry, University of California, Los Angeles, CA, USA.
Ali KhademhosseiniBiomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
Songtao ShiSchool of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Alireza MoshaveriniaWeintraub Center for Reconstructive Biotechnology, Division of Advanced Prothodontics, School of Dentistry, University of California, Los Angeles, CA, USA. amoshaverinia@ucla.edu.ORCID 0000-0002-1542-6202

Funding

OSTEOGENIC MECHANISMS OF SHEDR01DE017449 · NIDCR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI SHI, SONGTAO · 2007 to 2017
$3.5M
A NOVEL STEM CELL BASED DELIVERY SYSTEM TO IMPROVE BONE TISSUE REGENERATIONK08DE023825 · NIDCR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MOSHAVERINIA, ALIREZA · 2014 to 2018
$619k
NIDCR NIH HHS K08 DE023825NIDCR NIH HHS L30 DE025403NIDCR NIH HHS R01 DE017449
6 · The paper itself

Abstract

Repair and regeneration of muscle tissue following traumatic injuries or muscle diseases often presents a challenging clinical situation. If a significant amount of tissue is lost the native regenerative potential of skeletal muscle will not be able to grow to fill the defect site completely. Dental-derived mesenchymal stem cells (MSCs) in combination with appropriate scaffold material, present an advantageous alternative therapeutic option for muscle tissue engineering in comparison to current treatment modalities available. To date, there has been no report on application of gingival mesenchymal stem cells (GMSCs) in three-dimensional scaffolds for muscle tissue engineering. The objectives of the current study were to develop an injectable 3D RGD-coupled alginate scaffold with multiple growth factor delivery capacity for encapsulating GMSCs, and to evaluate the capacity of encapsulated GMSCs to differentiate into myogenic tissue in vitro and in vivo where encapsulated GMSCs were transplanted subcutaneously into immunocompromised mice. The results demonstrate that after 4 weeks of differentiation in vitro, GMSCs as well as the positive control human bone marrow mesenchymal stem cells (hBMMSCs) exhibited muscle cell-like morphology with high levels of mRNA expression for gene markers related to muscle regeneration (MyoD, Myf5, and MyoG) via qPCR measurement. Our quantitative PCR analyzes revealed that the stiffness of the RGD-coupled alginate regulates the myogenic differentiation of encapsulated GMSCs. Histological and immunohistochemical/fluorescence staining for protein markers specific for myogenic tissue confirmed muscle regeneration in subcutaneous transplantation in our in vivo animal model. GMSCs showed significantly greater capacity for myogenic regeneration in comparison to hBMMSCs (p < 0.05). Altogether, our findings confirmed that GMSCs encapsulated in RGD-modified alginate hydrogel with multiple growth factor delivery capacity is a promising candidate for muscle tissue engineering.

Indexed as

Tissue EngineeringAlginatesAnimalsCells, ImmobilizedFemaleGingivaGlucuronic AcidHexuronic AcidsHydrogelsIntercellular Signaling Peptides and ProteinsMesenchymal Stem CellsMiceMice, NudeMuscle, SkeletalOligopeptidesTissue ScaffoldsAlginatesarginyl-glycyl-aspartic acidGlucuronic AcidHexuronic AcidsHydrogelsIntercellular Signaling Peptides and ProteinsOligopeptidesDental mesenchymal stem cellsMuscle regenerationRGD-coupled alginate hydrogelTissue engineering

Identifiers

PMID27009085
PMCPMC4880526

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.