ArticleThe Journal of biological chemistry2016
Allicin Induces Thiol Stress in Bacteria through S-Allylmercapto Modification of Protein Cysteines.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 161 citations in OpenAlex.
- Article
- Cyclic Thiosulfinates: Synthesis and Applications in Chemical Biology and Materials Science.Chembiochem : a European journal of chemical biology · 2026Review
- Inhalable Spray-Dried Carriers for Deep Lung Delivery and In Situ Synthesis of Allicin.ACS omega · 2026Article
- Enhancing Allicin Purity and Gastrointestinal Bioactivity Profile of Garlic Extracts Through Optimized Supercritical-COFoods (Basel, Switzerland) · 2026Article
- Allium-Derived Compounds Against Multidrug-Resistant Bacteria: Antibacterial Activity and Synergistic Effects.ChemMedChem · 2026Article
- Effect of the chives, pomegranate, and lime juices on the meat quality and microbial reduction of chicken breast inoculated with Salmonella Enteritidis.Food science of animal resources · 2026Article
- Natural Antimicrobials from Plants Used as Food Preservatives.Foods (Basel, Switzerland) · 2026Review
- A Stepwise, Nitrosonium-Catalyzed Aerobic Oxidation of Thiols to Disulfides and Thiosulfonates.The Journal of organic chemistry · 2026Article
- The AltR transcription factor responds to plant thiosulfinates to regulate gene expression in a bacterial pathogen of onion.PLoS pathogens · 2026Article
- Organosulfur compounds as dual-action agents: a critical review of antimicrobial and immunomodulatory potentials, and translational barriers.Frontiers in pharmacology · 2026Review
- Modification of human metallothioneins by garlic organosulfur compounds, allicin and ajoene: direct effect on zinc homeostasis with relevance to immune regulation.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- Optimization of (Dithioperoxo)thiolate-Based Antifungal Agents for Triazole-ResistantPathogens (Basel, Switzerland) · 2025Article
- NLP-like deep learning aided in identification and validation of thiosulfinate tolerance clusters in diverse bacteria.mSphere · 2025Article
- In Search of New Drugs: Elucidating the Activity of Structurally Similar Potential Antibiotics Using Molecular Modelling.Molecules (Basel, Switzerland) · 2025Article
- Fluorinated Analogs of Organosulfur Compounds from Garlic (Molecules (Basel, Switzerland) · 2025Article
- Allicin enhances urea-N conversion to microbial-N by inhibiting urease activity and modulating the rumen microbiome in cattle.Microbiome · 2025Article
- Redox Mechanisms Driving Skin Fibroblast-to-Myofibroblast Differentiation.Antioxidants (Basel, Switzerland) · 2025Article
- Therapeutic potential of garlic, aged garlic extract and garlic‑derived compounds on pancreatic cancer (Review).Biomedical reports · 2025Review
- Harnessing Therapeutic Potential of Allicin Against Cancer: An Exploratory Review.Anti-cancer agents in medicinal chemistry · 2025Review
- Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Allicin (diallyl thiosulfinate) from garlic is a highly potent natural antimicrobial substance. It inhibits growth of a variety of microorganisms, among them antibiotic-resistant strains. However, the precise mode of action of allicin is unknown. Here, we show that growth inhibition of Escherichia coli during allicin exposure coincides with a depletion of the glutathione pool and S-allylmercapto modification of proteins, resulting in overall decreased total sulfhydryl levels. This is accompanied by the induction of the oxidative and heat stress response. We identified and quantified the allicin-induced modification S-allylmercaptocysteine for a set of cytoplasmic proteins by using a combination of label-free mass spectrometry and differential isotope-coded affinity tag labeling of reduced and oxidized thiol residues. Activity of isocitrate lyase AceA, an S-allylmercapto-modified candidate protein, is largely inhibited by allicin treatment in vivo Allicin-induced protein modifications trigger protein aggregation, which largely stabilizes RpoH and thereby induces the heat stress response. At sublethal concentrations, the heat stress response is crucial to overcome allicin stress. Our results indicate that the mode of action of allicin is a combination of a decrease of glutathione levels, unfolding stress, and inactivation of crucial metabolic enzymes through S-allylmercapto modification of cysteines.
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