ArticleJournal of applied oral science : revista FOB
Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds.
Article in Journal of applied oral science : revista FOB. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- The effect of liposomal ellagic acid on the biological properties of dental restorative materials: An in vitro comparative study.Biochemistry and biophysics reports · 2026Article
- Engineered biomaterial-based scaffolds for dentin-pulp complex regeneration: applications and biological performance-a scoping review.Odontology · 2026Review
- Optimizing methods for regenerative endodontics.Regenerative medicine · 2025Review
- Optimization of dental adhesive interfaces using tissue biomodulation with DESIGNER biopolymers.Dental materials : official publication of the Academy of Dental Materials · 2025Article
- Camellia Sinensis Extracts Embedded in Semipermeable Nanocapsules as Active Ingredient for Dental Adhesives: Biomaterial Synthesis and Effect on the Bond to Human Dentin.International journal of nanomedicine · 2025Article
- Antioxidants and Collagen-Crosslinking: Benefit on Bond Strength and Clinical Applicability.Materials (Basel, Switzerland) · 2020Article
- Polyphenols in Dental Applications.Bioengineering (Basel, Switzerland) · 2020Review
- Effects of proanthocyanidin, a crosslinking agent, on physical and biological properties of collagen hydrogel scaffold.Restorative dentistry & endodontics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe purpose of this study was to investigate the biological effects of epicatechin (ECN), a crosslinking agent, on human dental pulp cells (hDPCs) cultured in collagen scaffolds. MATERIAL AND
methodTo evaluate the effects of ECN on the proliferation of hDPCs, cell counting was performed using optical and fluorescent microscopy. Measurements of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reactions were performed to assess odontogenic differentiation. The compressive strength and setting time of collagen scaffolds containing ECN were measured. Differential scanning calorimetry was performed to analyze the thermal behavior of collagen in the presence of ECN.
resultsEpicatechin increased ALP activity, mineralized nodule formation, and the mRNA expression of dentin sialophosphoprotein (DSPP), a specific odontogenic-related marker. Furthermore, ECN upregulated the expression of DSPP in hDPCs cultured in collagen scaffolds. Epicatechin activated the extracellular signal-regulated kinase (ERK) and the treatment with an ERK inhibitor (U0126) blocked the expression of DSPP. The compressive strength was increased and the setting time was shortened in a dose-dependent manner. The number of cells cultured in the ECN-treated collagen scaffolds was significantly increased compared to the cells in the untreated control group.
conclusionsOur results revealed that ECN promoted the proliferation and differentiation of hDPCs. Furthermore, the differentiation was regulated by the ERK signaling pathway. Changes in mechanical properties are related to cell fate, including proliferation and differentiation. Therefore, our study suggests the ECN treatment might be desirable for dentin-pulp complex regeneration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.