ArticlePloS one2016
Earth Mover's Distance (EMD): A True Metric for Comparing Biomarker Expression Levels in Cell Populations.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed.
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- Phenoscaping Reveals Multimodal γδ T-cell Cytotoxicity as a Strategy to Overcome Cancer Cell-Mediated Immunomodulation.Cancer research · 2025Article
- Functional avidity of anti-B7H3 CAR-T constructs predicts antigen density thresholds for triggering effector function.Nature communications · 2025Article
- Gene trajectory inference for single-cell data by optimal transport metrics.Nature biotechnology · 2025Article
- Discovering genetic modulators of the protein homeostasis system through multilevel analysis.PNAS nexus · 2025Article
- Computational identification of surface markers for isolating distinct subpopulations from heterogeneous cancer cell populations.NPJ systems biology and applications · 2024Article
- Visualizing scRNA-Seq data at population scale with GloScope.Genome biology · 2024Article
- Visualizing scRNA-Seq Data at Population Scale with GloScope.bioRxiv : the preprint server for biology · 2024Article
- Computational identification of surface markers for isolating distinct subpopulations from heterogeneous cancer cell populations.bioRxiv : the preprint server for biology · 2024Article
- Evolutionary and developmental specialization of foveal cell types in the marmoset.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1.Neuron · 2024Article
- Speech Reception in Young Children with Autism Is Selectively Indexed by a Neural Oscillation Coupling Anomaly.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Computational Methods for Single-Cell Proteomics.Annual review of biomedical data science · 2023Review
- Activation priming and cytokine polyfunctionality modulate the enhanced functionality of low-affinity CD19 CAR T cells.Blood advances · 2023Article
- Single-cell analysis reveals inflammatory interactions driving macular degeneration.Nature communications · 2023Article
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14 authors.
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Abstract
Changes in the frequencies of cell subsets that (co)express characteristic biomarkers, or levels of the biomarkers on the subsets, are widely used as indices of drug response, disease prognosis, stem cell reconstitution, etc. However, although the currently available computational "gating" tools accurately reveal subset frequencies and marker expression levels, they fail to enable statistically reliable judgements as to whether these frequencies and expression levels differ significantly between/among subject groups. Here we introduce flow cytometry data analysis pipeline which includes the Earth Mover's Distance (EMD) metric as solution to this problem. Well known as an informative quantitative measure of differences between distributions, we present three exemplary studies showing that EMD 1) reveals clinically-relevant shifts in two markers on blood basophils responding to an offending allergen; 2) shows that ablative tumor radiation induces significant changes in the murine colon cancer tumor microenvironment; and, 3) ranks immunological differences in mouse peritoneal cavity cells harvested from three genetically distinct mouse strains.
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