ArticlePloS one2016
Does Embryo Culture Medium Influence the Health and Development of Children Born after In Vitro Fertilization?
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.
- Do IVF culture conditions have an impact on neonatal outcomes? A systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2024Pooled it
- Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials.Journal of assisted reproduction and genetics · 2016Pooled it
- Excess glutamine in embryo culture media alters mitochondrial and autophagy-related dynamics associated with impaired postnatal development.Frontiers in cell and developmental biology · 2026Article
- Association Between Human Embryo Culture Conditions, Cryopreservation, and the Potential Risk of Birth Defects in Children Conceived Through Assisted Reproduction Technology.Medicina (Kaunas, Lithuania) · 2025Review
- Transcriptomic effects of alginate hydrogel applied to the production of bovine embryos.Heliyon · 2024Article
- 3D culture applied to reproduction in females: possibilities and perspectives.Animal reproduction · 2024Review
- Can Cryopreservation in Assisted Reproductive Technology (ART) Induce Epigenetic Changes to Gametes and Embryos?Journal of clinical medicine · 2023Review
- Opportunities involving microfluidics and 3D culture systems to theAnimal reproduction · 2023Review
- Assessing the influence of distinct culture media on human pre-implantation development using single-embryo transcriptomics.Frontiers in cell and developmental biology · 2023Article
- Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.PloS one · 2023Article
- At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale.Human reproduction (Oxford, England) · 2022Article
- Culture medium is associated with the risks of placenta previa and macrosomia in pregnancies after in vitro fertilization.Archives of gynecology and obstetrics · 2022Article
- Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences.NPJ genomic medicine · 2022Article
- Genome-Wide Analysis of DNA Methylation in Buccal Cells of Children Conceived through IVF and ICSI.Genes · 2021Article
- Euploidy rates are not affected when embryos are cultured in a continuous (CCM) or sequential culture medium (SCM): a sibling oocyte study.Journal of assisted reproduction and genetics · 2021Observational
- Extracellular vesicles and its advances in female reproduction.Animal reproduction · 2020Article
- Increased blood pressure and impaired endothelial function after accelerated growth in IVF/ICSI children.Human reproduction open · 2020Article
- Which Low-Abundance Proteins are Present in the Human Milieu of Gamete/Embryo Maternal Interaction?International journal of molecular sciences · 2019Article
- Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life.Biochimica et biophysica acta. Molecular basis of disease · 2018Article
- No effect of IVF culture medium on cognitive development of 9-year-old children.Human reproduction open · 2018Article
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In animal studies, extensive data revealed the influence of culture medium on embryonic development, foetal growth and the behaviour of offspring. However, this impact has never been investigated in humans. For the first time, we investigated in depth the effects of embryo culture media on health, growth and development of infants conceived by In Vitro Fertilization until the age of 5 years old. This single-centre cohort study was based on an earlier randomized study. During six months, in vitro fertilization attempts (No. 371) were randomized according to two media (Single Step Medium--SSM group) or Global medium (Global group). This randomized study was stopped prematurely as significantly lower pregnancy and implantation rates were observed in the SSM group. Singletons (No. 73) conceived in the randomized study were included (42 for Global and 31 for SSM). The medical data for gestational, neonatal and early childhood periods were extracted from medical records and parental interviews (256 variables recorded). The developmental profiles of the children in eight domains (social, self-help, gross motor, fine motor, expressive language, language comprehension, letter knowledge and number knowledge--270 items) were compared in relation to the culture medium. The delivery rate was significantly lower in the SSM group than in the Global group (p<0.05). The culture medium had no significant effect on birthweight, risk of malformation (minor and major), growth and the frequency of medical concerns. However, the children of the Global group were less likely than those of the SSM group to show developmental problems (p = 0.002), irrespective of the different domains. In conclusion, our findings showed that the embryo culture medium may have an impact on further development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.