Evidence map›Paper›PMID 27007880›Full record

ArticleVirologica Sinica2016

Stability of HIV-1 subtype B and C Tat is associated with variation in the carboxyl-terminal region.

Xuechao Zhao, Lingyu Qian, Deyu Zhou, Di Qi, Chang Liu, Xiaohong Kong

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virologica Sinica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Barriers to HIV remission research in low- and middle-income countries.Journal of the International AIDS Society · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Xuechao ZhaoLaboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, 300071, China.
Lingyu QianLaboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, 300071, China.
Deyu ZhouLaboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, 300071, China.
Di QiLaboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, 300071, China.
Chang LiuLaboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, 300071, China.
Xiaohong KongLaboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, 300071, China. kongxh@nankai.edu.cn.
Nankai University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C worldwide. We hypothesized that amino acid differences contribute to functional differences among Tat proteins. In the present study, we found that subtype B NL4-3 Tat and subtype C isolate HIV1084i Tat exhibited differences in stability by overexpressing the fusion protein Tat-Flag. In addition, 1084i Tat can activate LTR and NF-κB more efficiently than NL4-3 Tat. In analyses of the activities of the truncated forms of Tat, we found that the carboxyl-terminal region of Tat regulates its stability and transactivity. According to our results, we speculated that the differences in stability between B-Tat and C-Tat result in differences in transactivation ability.

Indexed as

Amino Acid SubstitutionHEK293 CellsHIV-1HumansNF-kappa BProtein StabilityRecombinant Fusion ProteinsSequence DeletionStructure-Activity Relationshiptat Gene Products, Human Immunodeficiency VirusTranscriptional ActivationVirus ReplicationNF-kappa BRecombinant Fusion Proteinstat Gene Products, Human Immunodeficiency Viruscarboxyl-terminal regionNF-κB activationstabilitysubtype B/CTattransactivation

Identifiers

PMID27007880
PMCPMC8193441
OpenAlexW2305620874

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.