ArticlePlant cell reports2016
Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Comprehensive benchmarking of genome editing quantification methods for plant applications.iScience · 2025Article
- Review
- An efficient CRISPR-Cas12a-mediated MicroRNA knockout strategy in plants.Plant biotechnology journal · 2025Article
- Boosting genome editing in plants with single transcript unit surrogate reporter systems.Plant communications · 2024Article
- Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system.Nature communications · 2023Article
- Loss-function mutants of OsCKX gene family based on CRISPR-Cas systems revealed their diversified roles in rice.The plant genome · 2023Article
- An efficient CRISPR-Cas12a promoter editing system for crop improvement.Nature plants · 2023Article
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice.Plant biotechnology journal · 2022Article
- CRISPR-Cas9 mediated OsMIR168a knockout reveals its pleiotropy in rice.Plant biotechnology journal · 2022Article
- A Simple, Robust, and Cost-effective Method for Genotyping Small-scale Mutations.Journal of clinical and translational pathology · 2022Article
- Improved plant cytosine base editors with high editing activity, purity, and specificity.Plant biotechnology journal · 2021Article
- Efficient deletion of multiple circle RNA loci by CRISPR-Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice.Plant biotechnology journal · 2021Article
- An Editing-Site-Specific PCR Method for Detection and Quantification ofFoods (Basel, Switzerland) · 2021Article
- CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges.Genes · 2021Review
- CRISPR-Cas technology based genome editing for modification of salinity stress tolerance responses in rice (Oryza sativa L.).Molecular biology reports · 2021Review
- Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.Nature communications · 2021Article
- Creation of male-sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two-line system for the hybrid production of rice (Oryza sativa L.).Plant biotechnology journal · 2021Article
- PAM-less plant genome editing using a CRISPR-SpRY toolbox.Nature plants · 2021Article
- Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9.Methods in molecular biology (Clifton, N.J.) · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA method based on DNA single-strand conformation polymorphism is demonstrated for effective genotyping of CRISPR/Cas9-induced mutants in rice. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) has been widely adopted for genome editing in many organisms. A large proportion of mutations generated by CRISPR/Cas9 are very small insertions and deletions (indels), presumably because Cas9 generates blunt-ended double-strand breaks which are subsequently repaired without extensive end-processing. CRISPR/Cas9 is highly effective for targeted mutagenesis in the important crop, rice. For example, homozygous mutant seedlings are commonly recovered from CRISPR/Cas9-treated calli. However, many current mutation detection methods are not very suitable for screening homozygous mutants that typically carry small indels. In this study, we tested a mutation detection method based on single-strand conformational polymorphism (SSCP). We found it can effectively detect small indels in pilot experiments. By applying the SSCP method for CRISRP-Cas9-mediated targeted mutagenesis in rice, we successfully identified multiple mutants of OsROC5 and OsDEP1. In conclusion, the SSCP analysis will be a useful genotyping method for rapid identification of CRISPR/Cas9-induced mutants, including the most desirable homozygous mutants. The method also has high potential for similar applications in other plant species.
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