ArticlePloS one2016
Hemocyanin from Shrimp Litopenaeus vannamei Has Antiproliferative Effect against HeLa Cell In Vitro.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Research Progress on Shrimp Allergens and Allergenicity Reduction Methods.Foods (Basel, Switzerland) · 2025Review
- Shrimp hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation.Scientific reports · 2024Article
- Assessment of theBiomedicines · 2023Article
- Health-Promoting Additives Supplemented in Inert Microdiets for Whiteleg Shrimp (Animals : an open access journal from MDPI · 2023Article
- Antitumor Activity of Bioactive Compounds fromPharmaceuticals (Basel, Switzerland) · 2023Article
- LvHemB1, a novel cationic antimicrobial peptide derived from the hemocyanin of Litopenaeus vannamei, induces cancer cell death by targeting mitochondrial voltage-dependent anion channel 1.Cell biology and toxicology · 2022Article
- Physiological and muscle tissue responses inFrontiers in physiology · 2022Article
- Protein Diversity and Immune Specificity of Hemocyanin From ShrimpFrontiers in immunology · 2021Article
- Article
- AMolecules (Basel, Switzerland) · 2018Article
- The effects of hemocyanin on T cells culturedOncology letters · 2018Article
- Identification of SNPs potentially related to immune responses and growth performance inPeerJ · 2018Article
- Article
- Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin.Cellular and molecular life sciences : CMLS · 2017Review
- Litopenaeus vannamei hemocyanin exhibits antitumor activity in S180 mouse model in vivo.PloS one · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemocyanin (HMC) has been shown to participate in multiple roles of immune defence. In this study, we investigated the antiproliferative effect and underpinning mechanism of HMC from Litopenaeus vannamei in vitro. Sulforhodamine B (SRB) assay indicated that HMC could dramatically inhibit the growth of HeLa cells, but not 293T cells under the same conditions. Moreover, typical morphological features of apoptosis in HeLa cells including the formation of apoptotic body-like vesicles, chromatin condensation and margination were observed by using 4, 6-diamidino-2- phenylindole dihydrochloride (DAPI) staining and fluorescence analysis. An apoptotic DNA ladder from 180 to 300 bp was also detected. Furthermore, 10 variation proteins associated with apoptosis pathway, viz. G3PDH isoforms 1/2 (G3PDH1/2), aldosereductase, ectodemal dysplasia receptor associated death receptor domain isoform CRA_a (EDARADD), heat shock 60kD protein 1 variant 1 (HSP60), heat shock 70kDa protein 5 precursor (HSP70), heat shock protein 90kDa beta member 1 precursor (HSP90), 14-3-3 protein ζ/δ, Ran and ubiquitin activating enzyme E1(UBE1), were identified from HMC-treated HeLa cells by the proteomic and quantitative real-time RT-PCR strategies. Importantly, the reactive oxygen species (ROS), mitochondrial membrane potential (Δψm) and caspase-9/3 activities were changed significantly in HMC-treated HeLa cells. Together, the data suggests that L. vannamei HMC mediates antiproliferative properties through the apoptosis mechanism involving the mitochondria triggered pathway.
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Registered trials
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