ArticleIUCrJ2016
In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells.
Article in IUCrJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Review
- Micro-structured polymer fixed targets for serial crystallography at synchrotrons and XFELs.IUCrJ · 2023Article
- Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review.Molecules (Basel, Switzerland) · 2019Review
- An outlook on using serial femtosecond crystallography in drug discovery.Expert opinion on drug discovery · 2019Review
- Towards in cellulo virus crystallography.Scientific reports · 2018Article
- A Bright Future for Serial Femtosecond Crystallography with XFELs.Trends in biochemical sciences · 2017Review
- A pipeline for structure determination of in vivo-grown crystals using in cellulo diffraction.Acta crystallographica. Section D, Structural biology · 2016Article
- Widening the reach of structural biology.IUCrJ · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. The observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.