ArticleGut pathogens2016
The isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis in Shandong province, China.
Article in Gut pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 26 citations in OpenAlex.
- Prevalence and molecular insights of Mycobacterium avium subsp. paratuberculosis in Sudanese Cattle: implications for control and public health.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Sheep challenged with sheep-derived type II Mycobacterium avium subsp. paratuberculosis: the first experimental model of paratuberculosis in China.BMC veterinary research · 2025Article
- Prevalence and Genotyping ofVeterinary sciences · 2025Article
- The Impact ofPathogens (Basel, Switzerland) · 2024Article
- Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China.Veterinary sciences · 2024Article
- Identification and Phylogenetic Analysis ofTransboundary and emerging diseases · 2023Article
- Identification of Mycobacterium avium subspecies paratuberculosis in sheep farms in Bayannaoer, Inner Mongolia, China (short communication).BMC veterinary research · 2022Article
- Bio-typing of Mycobacterium avium subspecies paratuberculosis isolates recovered from the Himalayan sheep and goats.Tropical animal health and production · 2021Article
- Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.PloS one · 2021Article
- Characteristics and Epidemiological Investigation of Paratuberculosis in Dairy Cattle in Tai'an, China.BioMed research international · 2020Article
- Article
- Rapid visual detection ofJournal of veterinary science · 2018Article
- Pathogenesis, Molecular Genetics, and Genomics ofFrontiers in veterinary science · 2017Review
- MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byFrontiers in immunology · 2017Article
- Examination ofPeerJ · 2016Article
- First molecular typing of Mycobacterium avium subspecies paratuberculosis identified in animal and human drinking water from dairy goat farms in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
- The seroprevalence ofIrish veterinary journalArticle
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycobacterium avium subspecies paratuberculosis (Map) causes Johne's disease in domestic and wild ruminants. It has been a debate that whether Map can cause Crohn's disease in human. To our knowledge there is no report about molecular characterization of Map in China, although several Map strains have been reported in other country. The objectives of this study was to know the recent prevalence of Johne's disease in dairy farms in Shandong province, and have a better understanding of genotypic distribution of Map in China.
methodsJohne's disease was detected from 1038 individuals in 19 dairy farms by ELISA. Map in fecal and milk specimens was identified by Ziehl-Neelsen staining and confirmed using PCR-REA. In addition, frozen sections of ileum and mesenteric lymph nodes from two Map shedding cows were performed to observe the histopathological changes. Next-generation sequencing technology was performed to get whole genome sequences.
resultA total of 121 (11.7 %) animals were positive for Map antibody from 1038 sera tested, and 11 (57.9 %) dairy herds were positive for Map antibody. Typically histopathologic changes were observed in mesenteric lymph nodes. We have successfully isolated two Map strains, which both were Map-C. The current genome-wide analysis showed that the genome size of our isolates are respectively 4,750,273 and 4,727,050 bp with a same G + C content of 69.3 %, and the numbers of single nucleotide polymorphisms (SNPs) against Map K-10 are respectively 292 and 296.
conclusionMap is a prevalent pathogen among dairy cattle in China. This study successfully isolated two Map strains from one Chinese dairy herd with signs of diarrhoea, and identified that the two isolates were both Map-C. Furthermore, these isolates were most closely related to Map K-10.
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