ArticleBiotechnology for biofuels2016
Inhibition of lignin-derived phenolic compounds to cellulase.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 237 citations in OpenAlex.
- Phenolic compound-mediated covalent crosslinking as a universal mechanism for cellulase deactivation during lignocellulose saccharification.Nature communications · 2026Article
- Utilization of lignocellulosic mushroom cultivation residues for bioethanol production via consolidated bioprocessing.World journal of microbiology & biotechnology · 2026Article
- Synergistic pretreatment: hybrid strategies for maximum lignocellulose valorization.Biodegradation · 2026Review
- Maximizing Populus tremula biomass conversion: synergistic pretreatment effects on sugars release and lignin recovery.Bioresources and bioprocessing · 2026Article
- Conventional and Novel Strategies for Cellulose Isolation from Nut Shells: A Review.Molecules (Basel, Switzerland) · 2025Review
- Enhanced physicochemical characteristics and biological activities of low-temperature ethylenediamine/urea pretreated lignin.Bioprocess and biosystems engineering · 2025Article
- Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.Biotechnology letters · 2024Article
- Biochemical and inhibitor analysis of recombinant cellobiohydrolases from Phanerochaete chrysosporium.Biotechnology for biofuels and bioproducts · 2024Article
- Enhanced enzymatic sugar production from corn stover by combination of water extraction and glycerol-assisted instant catapult steam explosion.Bioresources and bioprocessing · 2024Article
- Biodetoxification of Lignocellulose Hydrolysate for Direct Use in Succinic Acid Production.Biodesign research · 2024Article
- Article
- Article
- Recent Advances in PLA-Based Antibacterial Food Packaging and Its Applications.Molecules (Basel, Switzerland) · 2022Review
- Bioprocess development of 2, 3-butanediol production using agro-industrial residues.Bioprocess and biosystems engineering · 2022Article
- Press water from the mechanical drying of Douglas-fir wood chips has multiple beneficial effects on lignocellulolytic fungi.Fungal biology and biotechnology · 2022Article
- Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains.Biotechnology for biofuels and bioproducts · 2022Review
- Improvement of Enzymatic Saccharification and Ethanol Production from Rice Straw Using Recycled Ionic Liquid: The Effect of Anti-Solvent Mixture.Bioengineering (Basel, Switzerland) · 2022Article
- Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland.PloS one · 2022Article
- Recent advances in understanding the effects of lignin structural characteristics on enzymatic hydrolysis.Biotechnology for biofuels · 2021Review
- Effect of lignin-blocking agent on enzyme hydrolysis of acid pretreated hemp waste.RSC advances · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLignin-derived phenolic compounds are universal in the hydrolysate of pretreated lignocellulosic biomass. The phenolics reduce the efficiency of enzymatic hydrolysis and increase the cost of ethanol production. We investigated inhibition of phenolics on cellulase during enzymatic hydrolysis using vanillin as one of the typical lignin-derived phenolics and Avicel as cellulose substrate.
resultsAs vanillin concentration increased from 0 to 10 mg/mL, cellulose conversion after 72-h enzymatic hydrolysis decreased from 53 to 26 %. Enzyme deactivation and precipitation were detected with the vanillin addition. The enzyme concentration and activity consecutively decreased during hydrolysis, but the inhibition degree, expressed as the ratio of the cellulose conversion without vanillin to the conversion with vanillin (A 0 /A), was almost independent on hydrolysis time. Inhibition can be mitigated by increasing cellulose loading or cellulase concentration. The inhibition degree showed linear relationship with the vanillin concentration and exponential relationship with the cellulose loading and the cellulase concentration. The addition of calcium chloride, BSA, and Tween 80 did not release the inhibition of vanillin significantly. pH and temperature for hydrolysis also showed no significant impact on inhibition degree. The presence of hydroxyl group, carbonyl group, and methoxy group in phenolics affected the inhibition degree.
conclusionBesides phenolics concentration, other factors such as cellulose loading, enzyme concentration, and phenolic structure also affect the inhibition of cellulose conversion. Lignin-blocking agents have little effect on the inhibition effect of soluble phenolics, indicating that the inhibition mechanism of phenolics to enzyme is likely different from insoluble lignin. The inhibition of soluble phenolics can hardly be entirely removed by increasing enzyme concentration or adding blocking proteins due to the dispersity and multiple binding sites of phenolics than insoluble lignin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.