Evidence map›Paper›PMID 27006454›Full record

ArticleStroke2016

Recombinant Osteopontin Stabilizes Smooth Muscle Cell Phenotype via Integrin Receptor/Integrin-Linked Kinase/Rac-1 Pathway After Subarachnoid Hemorrhage in Rats.

Jiang Wu, Yang Zhang, Peng Yang, Budbazar Enkhjargal, Anatol Manaenko, Jiping Tang, William J Pearce, Richard Hartman, Andre Obenaus, Gang Chen and 1 more

Expression of concernOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stroke, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
10.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 62 citations in OpenAlex.

  1. Review
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  16. Exendin-4 Preserves Blood-Brain Barrier IntegrityFrontiers in molecular neuroscience · 2021
    Article
  17. Review
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  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Jiang WuFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Yang ZhangFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Peng YangFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Budbazar EnkhjargalFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Anatol ManaenkoFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Jiping TangFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
William J PearceFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Richard HartmanFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Andre ObenausFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA.
Gang ChenFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA. johnzhang3910@yahoo.com nju_neurosurgery@163.com.
John H ZhangFrom the Department of Neurosurgery (J.W., G.C.), the First Affiliated Hospital of Soochow University, Suzhou, China; and Department of Physiology (J.W., Y.Z., P.Y., B.E., A.M., J.T., W.J.P., R.H., A.O., J.H.Z.), School of Behavioral Science (R.H.), Department of Pediatrics (A.O.), and Department of Anesthesiology (J.H.Z.), Loma Linda University, CA. johnzhang3910@yahoo.com nju_neurosurgery@163.com.
First Affiliated Hospital of Soochow University · CNSoochow University · CN

Funding

Vascular Biology Core FacilityP01NS082184 · NINDS · LOMA LINDA UNIVERSITY · PI PEARCE, WILLIAM J. · 2014 to 2018
$6.1M
Crotalus Snake Venom Preconditioning to Prevent Surgical Brain InjuryR01NS084921 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2014 to 2018
$1.7M
Neurovascular Protection for Early Brain Injury after SAHR01NS081740 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2013 to 2017
$1.6M
Cerebrovascular Myosin Light Chain Phosphorylation in Fetus, Newborn, and AdultR01NS076945 · NINDS · LOMA LINDA UNIVERSITY · PI PEARCE, WILLIAM J. · 2011 to 2015
$1.5M
NINDS NIH HHS P01 NS082184NINDS NIH HHS R01 NS076945NINDS NIH HHS R01 NS081740NINDS NIH HHS R01 NS084921
6 · The paper itself

Abstract

background and purposeRecombinant osteopontin (rOPN) has been reported to be neuroprotective in stroke animal models. The purpose of this study is to investigate a potential role and mechanism of nasal administration of rOPN on preserving the vascular smooth muscle phenotype in early brain injury after subarachnoid hemorrhage (SAH).

methodsOne hundred and ninety-two male adult Sprague-Dawley rats were used. The SAH model was induced by endovascular perforation. Integrin-linked kinase small interfering RNA was intracerebroventricularly injected 48 hours before SAH. The integrin receptor antagonist fibronectin-derived peptide Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP), focal adhesion kinase inhibitor Fib-14, and Rac-1 inhibitor NSC23766 were administered 1 hour before SAH induction. rOPN was administered via the intracerebroventricular and nasal route after SAH. SAH grade, neurological scores, brain water content, brain swelling, hematoxylin and eosin staining, India ink angiography, Western blots, and immunofluorescence were used to study the mechanisms of rOPN on the vascular smooth muscle phenotypic transformation.

resultsThe marker proteins of vascular smooth muscle phenotypic transformation α-smooth muscle actin decreased and embryonic smooth muscle myosin heavy chain (SMemb) increased significantly at 24 and 72 hours in the cerebral arteries after SAH. rOPN prevented the changes of α-smooth muscle actin and SMemb and significantly alleviated neurobehavioral dysfunction, increased the cross-sectional area and the lumen diameter of the cerebral arteries, reduced the brain water content and brain swelling, and improved the wall thickness of cerebral arteries. These effects of rOPN were abolished by GRGDSP, integrin-linked kinase small interfering RNA, and NSC23766. Intranasal application of rOPN at 3 hours after SAH also reduced neurological deficits.

conclusionsrOPN prevented the vascular smooth muscle phenotypic transformation and improved the neurological outcome, which was possibly mediated by the integrin receptor/integrin-linked kinase/Rac-1 pathway.

Indexed as

AnimalsCerebral ArteriesDisease Models, AnimalIntegrinsMaleMuscle, Smooth, VascularMyocytes, Smooth MuscleNeuroprotective AgentsOsteopontinPhenotypeProtein Serine-Threonine Kinasesrac1 GTP-Binding ProteinRatsRats, Sprague-DawleyRecombinant ProteinsScaffold Protein ILKIntegrinsNeuroprotective AgentsOsteopontinProtein Serine-Threonine Kinasesrac1 GTP-Binding ProteinRecombinant ProteinsScaffold Protein ILKblotting, Westerncerebral arteriesmusclerats, Sprague-Dawleysmooth, vascularsubarachnoid hemorrhage

Identifiers

PMID27006454
PMCPMC4846549
OpenAlexW2310243407

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.