Evidence map›Paper›PMID 27005473›Full record

ArticleBMC infectious diseases2016

Molecular Characterization of Giardia duodenalis in Children in Kenya.

C Mbae, E Mulinge, F Guleid, J Wainaina, A Waruru, Z K Njiru, S Kariuki

Open access · goldAbstract read
In one paragraph

Article in BMC infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 2 pooled it
4.0field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 38 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  6. Prevalence and Genetic Diversity ofJournal of parasitology research · 2025
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  8. Assemblages and Subassemblages ofJournal of parasitology research · 2024
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  13. The controversies surroundingCurrent research in parasitology & vector-borne diseases · 2021
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  18. Prevalence and genotypic characterization ofFood and waterborne parasitology · 2020
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  19. Slums, Space, and State of Health-A Link between Settlement Morphology and Health Data.International journal of environmental research and public health · 2020
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

C MbaeCentre for Microbiological Research, KEMRI, P.O Box 19464-00202, Nairobi, Kenya. cmkathure@gmail.com.
E MulingeCentre for Microbiological Research, KEMRI, P.O Box 19464-00202, Nairobi, Kenya.
F GuleidCentre for Microbiological Research, KEMRI, P.O Box 19464-00202, Nairobi, Kenya.
J WainainaInternational Livestock Research Institute, Naivasha Rd, P.O Box 30709, Nairobi, Kenya.
A WaruruKenya Medical Research Institute, P. O Box 58540-00200, Nairobi, Kenya.
Z K NjiruMurdoch University, School of Health Professions, Peel Campus, Mandurah, WA, 6210, Australia.
S KariukiCentre for Microbiological Research, KEMRI, P.O Box 19464-00202, Nairobi, Kenya.
Kenya Medical Research Institute · KEInternational Livestock Research Institute · KEMurdoch University · AU

Funding

Wellcome TrustWellcome Trust 084538
6 · The paper itself

Abstract

backgroundGiardia duodenalis is an important intestinal protozoan in humans worldwide with high infection rates occurring in densely populated and low resource settings. The parasite has been recorded to cause diarrhea in children. This study was carried out to identify G. duodenalis assemblages and sub-assemblages in children presenting with diarrhea in Kenya.

methodsA total of 2112 faecal samples were collected from children aged ≤ 5 years and screened for the presence of Giardia cysts using microscopy. A total of 96 (4.5%) samples were identified as Giardia positive samples and were genotyped using glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and β-giardin loci.

resultsThe three markers successfully genotyped 72 isolates and grouped 2 (1.4) isolates as Assemblage A, 64 (88.9) as Assemblage B and 7 (9.7%) consisted of mixed infections with assemblage A and B. A further analysis of 50 isolates using GDH Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) categorized 2 assemblage A isolates as sub-assemblage AII while 6 and 14 assemblage B isolates were categorized into sub-assemblage BIII and BIV respectively. A mixed infection with sub-assemblage BIII and BIV was recorded in 28 isolates. Over half (55.6%) of Giardia infections were recorded among the children between 13 to 48 months old.

conclusionThis paper reports the first data on the assemblages and sub-assemblages of Giardia duodenalis in children representing with diarrhea in Kenya.

Indexed as

Child Health ServicesChild, PreschoolDiarrheaFecesFemaleGenotypeGiardiaGiardiasisGlutamate DehydrogenaseHumansInfantInfant, NewbornKenyaMalePolymerase Chain ReactionPolymorphism, Restriction Fragment LengthGlutamate DehydrogenaseTriose-Phosphate IsomeraseChildrenGenotypingGiardiaInformal settlementsKenyaSubtyping

Identifiers

PMID27005473
PMCPMC4802924
OpenAlexW2305742138

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.