ArticleNature communications2016
Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Laboratory-based cellular-level correlative visible-light and X-ray microscopy for 3D evaluation of mouse kidney biopsy.Scientific reports · 2026Article
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- New Insight into Bone Immunity in Marrow Cavity and Cancellous Bone Microenvironments and Their Regulation.Biomedicines · 2025Review
- Article
- Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples.Nature biomedical engineering · 2024Article
- Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics.Npj imaging · 2024Review
- Millimetric scale two-photon Bessel-Gauss beam light sheet microscopy with three-axis isotropic resolution using an axicon lens.Neurophotonics · 2023Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Axial scanning of dual focus to improve light sheet microscopy.Biomedical optics express · 2022Article
- Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy.Biomedical optics express · 2021Article
- CUBIC-Cloud provides an integrative computational framework toward community-driven whole-mouse-brain mapping.Cell reports methods · 2021Article
- Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues.STAR protocols · 2021Article
- Tutorial: practical considerations for tissue clearing and imaging.Nature protocols · 2021Review
- A deep learning algorithm for 3D cell detection in whole mouse brain image datasets.PLoS computational biology · 2021Article
- Axial localization and tracking of self-interference nanoparticles by lateral point spread functions.Nature communications · 2021Article
- Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus.Frontiers in cell and developmental biology · 2021Article
- High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution.Journal of biomedical optics · 2020Article
- Planar Airy beam light-sheet for two-photon microscopy.Biomedical optics express · 2020Article
- Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.Annual review of biomedical engineering · 2020Review
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.