ArticleMicrobiologyOpen2016
Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.
Article in MicrobiologyOpen, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Diverse Genome Structures among Eukaryotes May Have Arisen in Response to Genetic Conflict.Genome biology and evolution · 2024Review
- Comprehensive analysis of flavohemoprotein copy number variation in Giardia intestinalis: exploring links to metronidazole resistance.Parasites & vectors · 2024Article
- Comparative genomics of Giardia duodenalis sub-assemblage AI beaver (Be-2) and human (WB-C6) strains show remarkable homozygosity, sequence similarity, and conservation of VSP genes.Scientific reports · 2024Article
- Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis.Genetics · 2024Review
- Giardia telomeres and telomerase.Parasitology research · 2024Review
- The adaptive roles of aneuploidy and polyclonality in Leishmania in response to environmental stress.EMBO reports · 2023Article
- The nucleolus of Giardia and its ribosomal biogenesis.Parasitology research · 2023Review
- Subspecific Nomenclature ofPathogens (Basel, Switzerland) · 2023Article
- Karyon: a computational framework for the diagnosis of hybrids, aneuploids, and other nonstandard architectures in genome assemblies.GigaScience · 2022Article
- Variation in haplotypes in single cysts of assemblages C and D, but not of assemblage E of Giardia duodenalis.BMC microbiology · 2022Article
- Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protistOpen biology · 2022Article
- Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.Nature communications · 2021Article
- Genetic Diversity of the Flavohemoprotein Gene ofInfection and drug resistance · 2020Article
- Tissue stem cells: the new actors in the aneuploidy field.Cell cycle (Georgetown, Tex.) · 2019Article
- The diverse consequences of aneuploidy.Nature cell biology · 2019Review
- Cellular Stress Associated with Aneuploidy.Developmental cell · 2018Review
- Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.MicrobiologyOpen · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia intestinalis is an important single-celled human pathogen. Interestingly, this organism has two equal-sized transcriptionally active nuclei, each considered diploid. By evaluating condensed chromosome numbers and visualizing homologous chromosomes by fluorescent in situ hybridization, we determined that the Giardia cells are constitutively aneuploid. We observed karyotype inter-and intra-population heterogeneity in eight cell lines from two clinical isolates, suggesting constant karyotype evolution during in vitro cultivation. High levels of chromosomal instability and frequent mitotic missegregations observed in four cell lines correlated with a proliferative disadvantage and growth retardation. Other cell lines, although derived from the same clinical isolate, revealed a stable yet aneuploid karyotype. We suggest that both chromatid missegregations and structural rearrangements contribute to shaping the Giardia genome, leading to whole-chromosome aneuploidy, unequal gene distribution, and a genomic divergence of the two nuclei within one cell. Aneuploidy in Giardia is further propagated without p53-mediated cell cycle arrest and might have been a key mechanism in generating the genetic diversity of this human pathogen.
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