ArticleThe Analyst2016
Single-cell mass spectrometry with multi-solvent extraction identifies metabolic differences between left and right blastomeres in the 8-cell frog (Xenopus) embryo.
Article in The Analyst, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 88 citations in OpenAlex.
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- The incorporation of MALDI mass spectrometry imaging in studies to identify markers of toxicity followingFrontiers in toxicology · 2024Article
- Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling.Research square · 2023Article
- Mass Spectrometry-based Single-Cell Lipidomics: Advancements, Challenges, and the Path Forward.Trends in analytical chemistry : TRAC · 2023Article
- Solute carrier (SLC) expression reveals skeletogenic cell diversity.Developmental biology · 2023Article
- The Yin and Yang of Breast Cancer: Ion Channels as Determinants of Left-Right Functional Differences.International journal of molecular sciences · 2023Article
- Single cell metabolism: current and future trends.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- Nodal asymmetry and hedgehog signaling during vertebrate left-right symmetry breaking.Frontiers in cell and developmental biology · 2022Article
- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology.Neuromethods · 2022Article
- Understanding laterality disorders and the left-right organizer: Insights from zebrafish.Frontiers in cell and developmental biology · 2022Review
- Temporal Gradients Controlling Embryonic Cell Cycle.Biology · 2021Review
- Altering metabolite distribution at Xenopus cleavage stages affects left-right gene expression asymmetries.Genesis (New York, N.Y. : 2000) · 2021Article
- Spray-Capillary-Based Capillary Electrophoresis Mass Spectrometry for Metabolite Analysis in Single Cells.Analytical chemistry · 2021Article
- Single cell metabolomics using mass spectrometry: Techniques and data analysis.Analytica chimica acta · 2021Review
- Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence.Frontiers in cell and developmental biology · 2021Review
- Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry inAnalytical chemistry · 2020Article
- Deciphering Metabolic Heterogeneity by Single-Cell Analysis.Analytical chemistry · 2019Article
- Integrating a generalized data analysis workflow with the Single-probe mass spectrometry experiment for single cell metabolomics.Analytica chimica acta · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Single-cell metabolic mass spectrometry enables the discovery (untargeted) analysis of small molecules in individual cells. Using single-cell capillary electrophoresis high-resolution mass spectrometry (CE-HRMS), we recently uncovered small-molecule differences between embryonic cells located along the animal-vegetal and dorsal-ventral axes of the 16-cell frog (Xenopus laevis) embryo, raising the question whether metabolic cell heterogeneity also exists along the left-right body axis. To address this question, we here advance single-cell CE-HRMS for identifying and quantifying metabolites in higher analytical sensitivity, and then use the methodology to compare metabolite production between left and right cells. Our strategy utilizes multiple solvents with complementary physicochemical properties to extract small molecules from single cells and improve electrophoretic separation, increasing metabolite ion signals for quantification and tandem HRMS. As a result, we were able to identify 55 different small molecules in D1 cells that were isolated from 8-cell embryos. To quantify metabolite production between left and right cells, we analyzed n = 24 different D1 cells in technical duplicate-triplicate measurements. Statistical and multivariate analysis based on 80 of the most repeatedly quantified compounds revealed 10 distinct metabolites that were significantly differentially accumulated in the left or right cells (p < 0.05 and fold change ≥1.5). These metabolites were enriched in the arginine-proline metabolic pathway in the right, but not the left D1 cells. Besides providing analytical benefits for single-cell HRMS, this work provides new metabolic data on the establishment of normal body asymmetry in the early developing embryo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.