Evidence map›Paper›PMID 27004464›Full record

ArticleScientific reports2016

Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1.

Chunsun Gu, Aiping Song, Xiaoxue Zhang, Haibin Wang, Ting Li, Yu Chen, Jiafu Jiang, Fadi Chen, Sumei Chen

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
8.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 41 citations in OpenAlex.

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  10. Genome-Wide Identification and Characterization of NAC Family inInternational journal of molecular sciences · 2022
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  11. Molecular Regulatory Networks for Improving Nitrogen Use Efficiency in Rice.International journal of molecular sciences · 2021
    Review
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  13. Article
  14. Article
  15. Knock-Down ofFrontiers in plant science · 2018
    Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Chunsun GuCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Aiping SongCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Xiaoxue ZhangCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Haibin WangCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Ting LiCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Yu ChenCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Jiafu JiangCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Fadi ChenCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Sumei ChenCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Nanjing Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) proteins which acts at low nitrate concentrations. The relevant gene content of the chrysanthemum genome was explored here by isolating the full length sequences of six distinct CmNRT2 genes. One of these (CmNRT2.1) was investigated at the functional level. Its transcription level was inducible by low concentrations of both nitrate and ammonium. A yeast two hybrid assay showed that CmNRT2.1 interacts with CmNAR2, while a BiFC assay demonstrated that the interaction occurs at the plasma membrane. Arabidopsis thaliana plants heterologously expressing CmNRT2.1 displayed an enhanced rate of labeled nitrogen uptake, suggesting that CmNRT2.1 represents a high affinity root nitrate transporter.

Indexed as

Amino Acid SequenceAmmonium CompoundsAnion Transport ProteinsArabidopsisCell MembraneChrysanthemumCloning, MolecularEvolution, MolecularGene Expression Regulation, PlantGenome, PlantNitratesNitrate TransportersNitrogenPhylogenyPlants, Genetically ModifiedAmmonium CompoundsAnion Transport ProteinsNitratesNitrate TransportersNitrogen

Identifiers

PMID27004464
PMCPMC4804277
OpenAlexW2304891860

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.