ArticleScientific reports2016
From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- S1 guideline for imaging diagnostics for skin diseases.Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG · 2025Guideline
- Imaging and quantifying drug delivery in skin - Part 2: Fluorescence andvibrational spectroscopic imaging methods.Advanced drug delivery reviews · 2020Pooled it
- CERS1 is a biomarker of Staphylococcus aureus abundance and atopic dermatitis severity.The Journal of allergy and clinical immunology · 2025Trial
- In vivo human skin imaging: Distinguishing cellular and fibrillar components with fluorescence lifetime microscopy.The Journal of investigative dermatology · 2026Article
- From Optical to Molecular Imaging on Human Skin: A Review.Chemical & biomedical imaging · 2026Review
- Intravital Visualization of Tattoo Particles After Picosecond Laser Treatment.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2025Observational
- Multiphoton tomographic analysis of hyaluronic acid delivery: comparison of carbon dioxide laser and 1927 nm thulium laser over time.Lasers in medical science · 2025Article
- Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view.Communications biology · 2025Article
- Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy.Light, science & applications · 2024Review
- Pixel-level classification of pigmented skin cancer lesions using multispectral autofluorescence lifetime dermoscopy imaging.Biomedical optics express · 2024Article
- Evaluation of compact pulsed lasers for two-photon microscopy using a simple method for measuring two-photon excitation efficiency.Neurophotonics · 2023Article
- Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies.Pharmaceutics · 2023Review
- Prognostic value of tumor necrosis based on the evaluation of frequency in invasive breast cancer.BMC cancer · 2023Article
- Multiphoton FLIM Analyses of Native and UVA-Modified Synthetic Melanins.International journal of molecular sciences · 2023Article
- Two-Photon Endoscopy: State of the Art and Perspectives.Molecular imaging and biology · 2023Review
- Article
- Discrimination of cancerous from benign pigmented skin lesions based on multispectral autofluorescence lifetime imaging dermoscopy and machine learning.Journal of biomedical optics · 2022Article
- Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions.Journal of biophotonics · 2021Review
- In vivo multiphoton imaging for non-invasive time course assessment of retinoids effects on human skin.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2020Article
- Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.
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Registered trials
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