ArticleDevelopment & reproduction2015
Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro.
Article in Development & reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells.Journal of visualized experiments : JoVE · 2021Article
- Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells.Journal of visualized experiments : JoVE · 2020Article
- The applicability of furfuryl-gelatin as a novel bioink for tissue engineering applications.Journal of biomedical materials research. Part B, Applied biomaterials · 2019Article
- Bioengineering functional smooth muscle with spontaneous rhythmic contraction in vitro.Scientific reports · 2018Article
- Human Pluripotent Stem Cell Culture: Current Status, Challenges, and Advancement.Stem cells international · 2018Review
- Visualization of morphological categories of colonies for monitoring of effect on induced pluripotent stem cell culture status.Regenerative therapy · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The suitable feeder cell layer is important for culture of embryonic stem (ES) cells. In this study, we investigated the effect of two kinds of the feeder cell, MEF cells and STO cells, layer to mouse ES (mES) cell culture for maintenance of stemness. We compare the colony formations, alkaline phosphatase (AP) activities, expression of pluripotency marker genes and proteins of D3 cell colonies cultured on MEF feeder cell layer (D3/MEF) or STO cell layers (D3/STO) compared to feeder free condition (D3/-) as a control group. Although there were no differences to colony formations and AP activities, interestingly, the transcripts level of pluripotency marker genes, Pou5f1 and Nanog were highly expressed in D3/MEF (79 and 93) than D3/STO (61and 77) or D3/- (65 and 81). Also, pluripotency marker proteins, NANOG and SOX-2, were more synthesized in D3/MEF (72.8±7.69 and 81.2±3.56) than D3/STO (32.0±4.30 and 56.0±4.90) or D3/- (55.0±4.64 and 62.0±6.20). These results suggest that MEF feeder cell layer is more suitable to mES cell culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.