ArticleeLife2016
A novel biosensor to study cAMP dynamics in cilia and flagella.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 86 citations in OpenAlex.
- Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools.Experimental & molecular medicine · 2025Review
- A molecular mechanism to diversify CaNature communications · 2024Article
- Uncovering cilia function in glial development.Annals of human genetics · 2024Review
- A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond).Frontiers in cell and developmental biology · 2024Article
- Emerging principles of primary cilia dynamics in controlling tissue organization and function.The EMBO journal · 2023Review
- Control of intracellular pH and bicarbonate by CONature communications · 2023Article
- Algal rhodopsins encoding diverse signal sequence holds potential for expansion of organelle optogenetics.Biophysics and physicobiology · 2023Article
- A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging.Nature communications · 2022Article
- A cAMP Sensor Based on Ligand-Dependent Protein Stabilization.ACS chemical biology · 2022Article
- Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Released ATP Mediates Spermatozoa Chemotaxis Promoted by Uterus-Derived Factor (UDF) inInternational journal of molecular sciences · 2022Article
- Control of the Hedgehog pathway by compartmentalized PKA in the primary cilium.Science China. Life sciences · 2022Review
- Imaging the cAMP Signaling Microdomain of the Primary Cilium Using Targeted FRET-Based Biosensors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening.Frontiers in pharmacology · 2022Article
- Review
- Article
- Molecular mechanisms of sperm motility are conserved in an early-branching metazoan.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Genetically encoded sensors towards imaging cAMP and PKA activity in vivo.Journal of neuroscience methods · 2021Review
- Soluble adenylyl cyclase inhibition prevents human sperm functions essential for fertilization.Molecular human reproduction · 2021Article
- An L-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR.Nature communications · 2021Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular messenger cAMP regulates multiple cellular functions, including signaling in cilia and flagella. The cAMP dynamics in these subcellular compartments are ill-defined. We introduce a novel FRET-based cAMP biosensor with nanomolar sensitivity that is out of reach for other sensors. To measure cAMP dynamics in the sperm flagellum, we generated transgenic mice and reveal that the hitherto methods determining total cAMP levels do not reflect changes in free cAMP levels. Moreover, cAMP dynamics in the midpiece and principal piece of the flagellum are distinctively different. The sole cAMP source in the flagellum is the soluble adenylate cyclase (SACY). Although bicarbonate-dependent SACY activity requires Ca(2+), basal SACY activity is suppressed by Ca(2+). Finally, we also applied the sensor to primary cilia. Our new cAMP biosensor features unique characteristics that allow gaining new insights into cAMP signaling and unravel the molecular mechanisms underlying ciliary function in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.