ArticleCell adhesion & migration2016
Selective binding and lateral clustering of α5β1 and αvβ3 integrins: Unraveling the spatial requirements for cell spreading and focal adhesion assembly.
Article in Cell adhesion & migration, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Modulating Integrin and Growth Factor Signaling With Peptides: Strategies to Synergistically Enhance Bone Tissue Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- CFTR dictates monocyte adhesion by facilitating integrin clustering but not activation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biological boundary conditions regulate the internalization ofFrontiers in cellular and infection microbiology · 2025Article
- Modelling how curved active proteins and shear flow pattern cellular shape and motility.Frontiers in cell and developmental biology · 2023Article
- Regulation of cardiomyocyte adhesion and mechanosignalling through distinct nanoscale behaviour of integrin ligands mimicking healthy or fibrotic extracellular matrix.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Critical adhesion areas of cells on micro-nanopatterns.Nano research · 2022Article
- Amphiphilic Copolymers for Versatile, Facile, and In Situ Tunable Surface Biofunctionalization.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells.Scientific reports · 2021Article
- ECM-Mimetic Nylon Nanofiber Scaffolds for Neurite Growth Guidance.Nanomaterials (Basel, Switzerland) · 2021Article
- Integrin Subtypes and Nanoscale Ligand Presentation Influence Drug Sensitivity in Cancer Cells.Nano letters · 2020Article
- Multiscale model of integrin adhesion assembly.PLoS computational biology · 2019Article
- Clustering of integrin β cytoplasmic domains triggers nascent adhesion formation and reveals a protozoan origin of the integrin-talin interaction.Scientific reports · 2019Article
- Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation.Stem cells international · 2019Review
- Mechanistic understanding of nanoparticles' interactions with extracellular matrix: the cell and immune system.Particle and fibre toxicology · 2017Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Coordination of the specific functions of α5β1 and αvβ3 integrins is crucial for the precise regulation of cell adhesion, spreading and migration, yet the contribution of differential integrin-specific crosstalk to these processes remains unclear. To determine the specific functions of αvβ3 and α5β1 integrins, we used nanoarrays of gold particles presenting immobilized, integrin-selective peptidomimetic ligands. Integrin binding to the peptidomimetics is highly selective, and cells can spread on both ligands. However, spreading is faster and the projected cell area is greater on α5β1 ligand; both depend on ligand spacing. Quantitative analysis of adhesion plaques shows that focal adhesion size is increased in cells adhering to αvβ3 ligand at 30 and 60 nm spacings. Analysis of αvβ3 and α5β1 integrin clusters indicates that fibrillar adhesions are more prominent in cells adhering to α5β1 ligand, while clusters are mostly localized at the cell margins in cells adhering to αvβ3 ligand. αvβ3 integrin clusters are more pronounced on αvβ3 ligand, though they can also be detected in cells adhering to α5β1 ligand. Furthermore, α5β1 integrin clusters are present in cells adhering to α5β1 ligand, and often colocalize with αvβ3 clusters. Taken together, these findings indicate that the activation of αvβ3 integrin by ligand binding is dispensable for initial adhesion and spreading, but essential to formation of stable focal adhesions.
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