ArticleThe Journal of cell biology2016
Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore.
Article in The Journal of cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- A Computational Pipeline for Quantifying Kinetochore Morphological Changes in Live Cells.bioRxiv : the preprint server for biology · 2026Article
- Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function.bioRxiv : the preprint server for biology · 2025Article
- Optimal strategies for correcting merotelic chromosome attachments in anaphase.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chromosome size-dependent polar ejection force impairs mammalian mitotic error correction.The Journal of cell biology · 2024Article
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Article
- Chromosome size-dependent polar ejection force impairs mammalian mitotic error correction.bioRxiv : the preprint server for biology · 2023Article
- Mechanical coupling coordinates microtubule growth.bioRxiv : the preprint server for biology · 2023Article
- Kinetochore life histories reveal an Aurora-B-dependent error correction mechanism in anaphase.Developmental cell · 2021Article
- Shake It Off: The Elimination of Erroneous Kinetochore-Microtubule Attachments and Chromosome Oscillation.International journal of molecular sciences · 2021Review
- Centromere mechanical maturation during mammalian cell mitosis.Nature communications · 2019Article
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
- Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.Molecular biology of the cell · 2018Article
- Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles.European biophysics journal : EBJ · 2018Review
- Mitotic tethers connect sister chromosomes and transmit "cross-polar" force during anaphase A of mitosis in PtK2 cells.Biomedical optics express · 2017Article
- Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.Current biology : CB · 2017Article
- Cutting edge science: Laser surgery illuminates viscoelasticity of merotelic kinetochores.The Journal of cell biology · 2016Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Accurate chromosome segregation depends on proper kinetochore-microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore, which is laterally stretched by opposing pulling forces exerted by microtubules, and inferred the mechanical response of the kinetochore from its length change. In both mammalian PtK1 cells and in the fission yeast Schizosaccharomyces pombe, kinetochores shortened after microtubule severing. Interestingly, the inner kinetochore-centromere relaxed faster than the outer kinetochore. Whereas in fission yeast all kinetochores relaxed to a similar length, in PtK1 cells the more stretched kinetochores remained more stretched. Simple models suggest that these differences arise because the mechanical structure of the mammalian kinetochore is more complex. Our study establishes merotelic kinetochores as an experimental model for studying the mechanical response of the kinetochore in live cells and reveals a viscoelastic behavior of the kinetochore that is conserved in yeast and mammalian cells.
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