ArticleProceedings of the National Academy of Sciences of the United States of America2016
Cell-specific localization of alkaloids in Catharanthus roseus stem tissue measured with Imaging MS and Single-cell MS.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 131 citations in OpenAlex.
- Single-Cell Insights into Medicinal Plant Development and Metabolism.Plants (Basel, Switzerland) · 2026Review
- Seasonal variation affects the accumulation of alkaloids in Amsonia elliptica.Journal of plant research · 2026Article
- Mass Spectrometry Imaging Combined With Single-Cell Transcriptional Profiling Reveals the Multidimensional Spatial Distributions and Biosynthetic Pathways of Medicinal Components in Andrographis paniculata.Plant biotechnology journal · 2026Article
- Integrating Single-Cell and Spatial Multi-Omics to Decode Plant-Microbe Interactions at Cellular Resolution.Microorganisms · 2026Review
- A cell-type-specific regulon controlling monoterpene indole alkaloid biosynthesis with feedback and feedforward activation loops.The New phytologist · 2026Article
- Spatial metabolomics as a new avenue in plant developmental biology: insights into serine biosynthesis during spermatogenesis inPlant signaling & behavior · 2025Article
- Single-cell metabolome and RNA-seq multiplexing on single plant cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Plant metabolism: Zoom in to the single-cell level.Plant physiology · 2025Review
- Transcriptional Reprogramming Deploys a Compartmentalized 'Timebomb' in Catharanthus roseus to Fend Off Chewing Herbivores.Plant, cell & environment · 2025Article
- The aerial epidermis is a major site of quinolizidine alkaloid biosynthesis in narrow-leafed lupin.The New phytologist · 2025Article
- Metabolic profiling and pharmacological evaluation of alkaloids in threeFrontiers in plant science · 2025Article
- Cell-type-aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.The New phytologist · 2025Article
- Quantitative Single-Cell Mass Spectrometry Provides a Highly Resolved Analysis of Natural Product Biosynthesis Partitioning in Plants.Journal of the American Chemical Society · 2024Article
- Characterization of a vacuolar importer of secologanin in Catharanthus roseus.Communications biology · 2024Article
- Application of single-cell multi-omics approaches in horticulture research.Molecular horticulture · 2023Review
- Advances in single-cell metabolomics to unravel cellular heterogeneity in plant biology.Plant physiology · 2023Review
- Single-cell multi-omics in the medicinal plant Catharanthus roseus.Nature chemical biology · 2023Article
- Genome-wide identification and expression analysis of TCP family genes inFrontiers in plant science · 2023Article
- Multimatrix Variation Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry as a Tool for Determining the Bonding of Nitrogen Atoms in Alkaloids.Journal of the American Society for Mass Spectrometry · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catharanthus roseus (L.) G. Don is a medicinal plant well known for producing antitumor drugs such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). The TIA metabolic pathway in C. roseus has been extensively studied. However, the localization of TIA intermediates at the cellular level has not been demonstrated directly. In the present study, the metabolic pathway of TIA in C. roseus was studied with two forefront metabolomic techniques, that is, Imaging mass spectrometry (MS) and live Single-cell MS, to elucidate cell-specific TIA localization in the stem tissue. Imaging MS indicated that most TIAs localize in the idioblast and laticifer cells, which emit blue fluorescence under UV excitation. Single-cell MS was applied to four different kinds of cells [idioblast (specialized parenchyma cell), laticifer, parenchyma, and epidermal cells] in the stem longitudinal section. Principal component analysis of Imaging MS and Single-cell MS spectra of these cells showed that similar alkaloids accumulate in both idioblast cell and laticifer cell. From MS/MS analysis of Single-cell MS spectra, catharanthine, ajmalicine, and strictosidine were found in both cell types in C. roseus stem tissue, where serpentine was also accumulated. Based on these data, we discuss the significance of TIA synthesis and accumulation in the idioblast and laticifer cells of C. roseus stem tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.