ArticleNature chemistry2016
Designed metalloprotein stabilizes a semiquinone radical.
Article in Nature chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 97 citations in OpenAlex.
- Structure-activity relationships in catalytic amyloids.Journal of inorganic biochemistry · 2026Article
- A cytokine-based designer enzyme with an abiological multinuclear metal center exhibits intrinsic and extrinsic catalysis.Nature communications · 2025Article
- Hydroxyl Radical-π Interaction in a Single Crystal.JACS Au · 2025Article
- De novo design of drug-binding proteins with predictable binding energy and specificity.Science (New York, N.Y.) · 2024Article
- Article
- Mechanisms and Opportunities for Rational In Silico Design of Enzymes to Degrade Per- and Polyfluoroalkyl Substances (PFAS).Journal of chemical information and modeling · 2023Review
- Indole-5,6-quinones display hallmark properties of eumelanin.Nature chemistry · 2023Article
- Designed Rubredoxin miniature in a fully artificial electron chain triggered by visible light.Nature communications · 2023Article
- Nanoantioxidant Materials: Nanoengineering Inspired by Nature.Micromachines · 2023Review
- Modulation of Slow Magnetic Relaxation in Gd(III)-Tetrahalosemiquinonate Complexes.Chemistry, an Asian journal · 2022Article
- The stability and dynamics of computationally designed proteins.Protein engineering, design & selection : PEDS · 2022Review
- A new regime of heme-dependent aromatic oxygenase superfamily.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecularly soldered covalent organic frameworks for ultrafast precision sieving.Science advances · 2021Article
- Effects of Ionic Liquids on Metalloproteins.Molecules (Basel, Switzerland) · 2021Review
- Allosteric cooperation in a de novo-designed two-domain protein.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.Angewandte Chemie (International ed. in English) · 2020Article
- Review
- Tuning the stability of organic radicals: from covalent approaches to non-covalent approaches.Chemical science · 2020Review
- Uno Ferro, a de novo Designed Protein, Binds Transition Metals with High Affinity and Stabilizes Semiquinone Radical Anion.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Rational Design of Artificial Metalloproteins and Metalloenzymes with Metal Clusters.Molecules (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Enzymes use binding energy to stabilize their substrates in high-energy states that are otherwise inaccessible at ambient temperature. Here we show that a de novo designed Zn(II) metalloprotein stabilizes a chemically reactive organic radical that is otherwise unstable in aqueous media. The protein binds tightly to and stabilizes the radical semiquinone form of 3,5-di-tert-butylcatechol. Solution NMR spectroscopy in conjunction with molecular dynamics simulations show that the substrate binds in the active site pocket where it is stabilized by metal-ligand interactions as well as by burial of its hydrophobic groups. Spectrochemical redox titrations show that the protein stabilized the semiquinone by reducing the electrochemical midpoint potential for its formation via the one-electron oxidation of the catechol by approximately 400 mV (9 kcal mol(-1)). Therefore, the inherent chemical properties of the radical were changed drastically by harnessing its binding energy to the metalloprotein. This model sets the basis for designed enzymes with radical cofactors to tackle challenging chemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.