ArticleScientific reports2016
Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Traumatic and Diabetic Schwann Cell Demyelination Is Triggered by a Transient Mitochondrial Calcium Release through Voltage Dependent Anion Channel 1.Biomedicines · 2022Article
- Defining Local Chemical Conditions in Magnetosomes of Magnetotactic Bacteria.The journal of physical chemistry. B · 2022Article
- Automated Quantification and Network Analysis of Redox Dynamics in Neuronal Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A comparison of Prx- and OxyR-based HThe Journal of biological chemistry · 2021Article
- Mitochondria at Work: New Insights into Regulation and Dysregulation of Cellular Energy Supply and Metabolism.Biomedicines · 2020Review
- New insights in the mechanisms of impaired redox signaling and its interplay with inflammation and immunity in multiple sclerosis.Physiological research · 2020Review
- Imaging pH Dynamics Simultaneously in Two Cellular Compartments Using a Ratiometric pH-Sensitive Mutant of mCherry.ACS omega · 2018Article
- 13 reasons why the brain is susceptible to oxidative stress.Redox biology · 2018Review
- Regulation of neuronal development and function by ROS.FEBS letters · 2018Review
- Guidelines on experimental methods to assess mitochondrial dysfunction in cellular models of neurodegenerative diseases.Cell death and differentiation · 2018Review
- Mitochondrial Heterogeneity.Frontiers in genetics · 2018Review
- Assessing Spatiotemporal and Functional Organization of Mitochondrial Networks.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Functional Implications of Cardiac Mitochondria Clustering.Advances in experimental medicine and biology · 2017Review
- Mitochondrial health, the epigenome and healthspan.Clinical science (London, England : 1979) · 2016Review
- Resolving Contributions of Oxygen-Consuming and ROS-Generating Enzymes at the Synapse.Oxidative medicine and cellular longevity · 2016Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
Redox switches are important mediators in neoplastic, cardiovascular and neurological disorders. We recently identified spontaneous redox signals in neurons at the single mitochondrion level where transients of glutathione oxidation go along with shortening and re-elongation of the organelle. We now have developed advanced image and signal-processing methods to re-assess and extend previously obtained data. Here we analyze redox and pH signals of entire mitochondrial populations. In total, we quantified the effects of 628 redox and pH events in 1797 mitochondria from intercostal axons and neuromuscular synapses using optical sensors (mito-Grx1-roGFP2; mito-SypHer). We show that neuronal mitochondria can undergo multiple redox cycles exhibiting markedly different signal characteristics compared to single redox events. Redox and pH events occur more often in mitochondrial clusters (medium cluster size: 34.1 ± 4.8 μm(2)). Local clusters possess higher mitochondrial densities than the rest of the axon, suggesting morphological and functional inter-mitochondrial coupling. We find that cluster formation is redox sensitive and can be blocked by the antioxidant MitoQ. In a nerve crush paradigm, mitochondrial clusters form sequentially adjacent to the lesion site and oxidation spreads between mitochondria. Our methodology combines optical bioenergetics and advanced signal processing and allows quantitative assessment of entire mitochondrial populations.
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Registered trials
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