ReviewParasitology2017
Transgenesis in Strongyloides and related parasitic nematodes: historical perspectives, current functional genomic applications and progress towards gene disruption and editing.
Review in Parasitology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 83 citations in OpenAlex.
- Generation ofJBMR plus · 2026Article
- Neuronally sensed oxygen drives behavior and development in human-infective, skin-penetrating nematodes.bioRxiv : the preprint server for biology · 2026Article
- Transgenesis and targeted mutagenesis in the human-parasitic nematodeFrontiers in parasitology · 2026Review
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- Aptazyme-mediated gene regulation in Strongyloides stercoralis for functional studies of insulin receptor isoform specificity.PLoS pathogens · 2025Article
- Current trends in application of CRISPR/Cas9 in gene editing and diagnostics in Neglected tropical diseases (NTDs).Molecular biology reports · 2025Review
- Carbon dioxide shapes parasite-host interactions in a human-infective nematode.Current biology : CB · 2025Article
- CRISPR-based functional genomics for schistosomes and related flatworms.Trends in parasitology · 2024Review
- The generation of stable transgenic lines in the human-infective nematode Strongyloides stercoralis.G3 (Bethesda, Md.) · 2024Article
- Systemic Immune Modulation by Gastrointestinal Nematodes.Annual review of immunology · 2024Review
- Introduction toJournal of nematology · 2024Article
- A standard workflow for community-driven manual curation ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Review
- Invade or die: behaviours and biochemical mechanisms that drive skin penetration inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Astacin metalloproteases in human-parasitic nematodes.Advances in parasitology · 2024Review
- Hookworms dynamically respond to loss of Type 2 immune pressure.PLoS pathogens · 2023Article
- A comparative 'omics' approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens.PLoS neglected tropical diseases · 2023Article
- Using newly optimized genetic tools to probe Strongyloides sensory behaviors.Molecular and biochemical parasitology · 2022Review
- The neural basis of heat seeking in a human-infective parasitic worm.Current biology : CB · 2022Article
- Transgenesis in parasitic helminths: a brief history and prospects for the future.Parasites & vectors · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Transgenesis for Strongyloides and Parastrongyloides was accomplished in 2006 and is based on techniques derived for Caenorhabditis elegans over two decades earlier. Adaptation of these techniques has been possible because Strongyloides and related parasite genera carry out at least one generation of free-living development, with adult males and females residing in soil contaminated by feces from an infected host. Transgenesis in this group of parasites is accomplished by microinjecting DNA constructs into the syncytia of the distal gonads of free-living females. In Strongyloides stercoralis, plasmid-encoded transgenes are expressed in promoter-regulated fashion in the F1 generation following gene transfer but are silenced subsequently. Stable inheritance and expression of transgenes in S. stercoralis requires their integration into the genome, and stable lines have been derived from integrants created using the piggyBac transposon system. More direct investigations of gene function involving expression of mutant transgene constructs designed to alter intracellular trafficking and developmental regulation have shed light on the function of the insulin-regulated transcription factor Ss-DAF-16. Transgenesis in Strongyloides and Parastrongyloides opens the possibility of powerful new methods for genome editing and transcriptional manipulation in this group of parasites. Proof of principle for one of these, CRISPR/Cas9, is presented in this review.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.