ArticlePloS one2016
Whole-Genome Saliva and Blood DNA Methylation Profiling in Individuals with a Respiratory Allergy.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 2 syntheses or guidelines pooled it, 56 citations in OpenAlex.
- Polymethylation scores for prenatal maternal smoke exposure persist until age 15 and are detected in saliva in the Fragile Families and Child Wellbeing cohort.Epigenetics · 2022Pooled it
- The Impact of Air Pollution on Our Epigenome: How Far Is the Evidence? (A Systematic Review).Current environmental health reports · 2018Pooled it
- Review
- The PM20D1-OLE pathway induces microglia rewiring to ameliorate Alzheimer disease.Cell death & disease · 2026Article
- Comparison of DNA methylation measured in saliva and peripheral blood as a marker of exposure and outcome: a scoping review.Clinical epigenetics · 2026Article
- Genetic impacts on within-pair DNA methylation variance in monozygotic twins capture gene-environment interactions and cell-type effects.Genome biology · 2026Article
- Genome-Wide Blood DNA Methylation Profiling in Birch Pollen Allergic Patients Undergoing Allergen-Specific Immunotherapy.Allergy · 2025Article
- Changes in DNA Methylation Associated With Psychobehavioral Interventions: A Scoping Literature Review.Biological research for nursing · 2025Article
- Biological Mechanisms for Allen's Rule: DNA Methylation as Mediator of the Association Between In Utero Exposure to Environmental Heat and Tibial Growth in Childhood.American journal of human biology : the official journal of the Human Biology Council · 2025Article
- Saliva as a potential diagnostic medium: DNA methylation biomarkers for disorders beyond the oral cavity.NPJ genomic medicine · 2025Article
- Salivary biomarkers in cancer detection and management.Acta biochimica Polonica · 2025Review
- Genetic and epigenetic biomarkers in human biomonitoring: why needed and how can Oxford Nanopore sequencing contribute?Frontiers in public health · 2025Review
- Mother's smoking habits affects IL10 methylation but not asthma in Ecuadorian children.Molecular genetics & genomic medicine · 2024Article
- Article
- Association of DNA methylation signatures with cognitive performance among smokers and ex-smokers.Tobacco induced diseases · 2023Article
- Microbial contamination and composition of oral samples subjected to clinical whole genome sequencing.Frontiers in genetics · 2023Article
- Article
- Disrupted methylation patterns at birth persist in early childhood: a prospective cohort analysis.Clinical epigenetics · 2022Article
- Review
- Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers.Biosensors · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The etiology of respiratory allergies (RA) can be partly explained by DNA methylation changes caused by adverse environmental and lifestyle factors experienced early in life. Longitudinal, prospective studies can aid in the unravelment of the epigenetic mechanisms involved in the disease development. High compliance rates can be expected in these studies when data is collected using non-invasive and convenient procedures. Saliva is an attractive biofluid to analyze changes in DNA methylation patterns. We investigated in a pilot study the differential methylation in saliva of RA (n = 5) compared to healthy controls (n = 5) using the Illumina Methylation 450K BeadChip platform. We evaluated the results against the results obtained in mononuclear blood cells from the same individuals. Differences in methylation patterns from saliva and mononuclear blood cells were clearly distinguishable (PAdj<0.001 and |Δβ|>0.2), though the methylation status of about 96% of the cg-sites was comparable between peripheral blood mononuclear cells and saliva. When comparing RA cases with healthy controls, the number of differentially methylated sites (DMS) in saliva and blood were 485 and 437 (P<0.05 and |Δβ|>0.1), respectively, of which 216 were in common. The methylation levels of these sites were significantly correlated between blood and saliva. The absolute levels of methylation in blood and saliva were confirmed for 3 selected DMS in the PM20D1, STK32C, and FGFR2 genes using pyrosequencing analysis. The differential methylation could only be confirmed for DMS in PM20D1 and STK32C genes in saliva. We show that saliva can be used for genome-wide methylation analysis and that it is possible to identify DMS when comparing RA cases and healthy controls. The results were replicated in blood cells of the same individuals and confirmed by pyrosequencing analysis. This study provides proof-of-concept for the applicability of saliva-based whole-genome methylation analysis in the field of respiratory allergy.
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