ArticlePloS one2016
A Zebrafish Larval Model to Assess Virulence of Porcine Streptococcus suis Strains.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Identification ofVirulence · 2021Pooled it
- Decadal advances and future prospects in subunit vaccine development againstFrontiers in immunology · 2025Review
- Diving into drug-screening: zebrafish embryos as an in vivo platform for antimicrobial drug discovery and assessment.FEMS microbiology reviews · 2024Review
- Article
- Comparative transcriptomic analysis reveal genes involved in the pathogenicity increase ofVirulence · 2022Article
- Screening for Virulence-Related Genes via a Transposon Mutant Library ofMicroorganisms · 2022Article
- The global emergence of a novel Streptococcus suis clade associated with human infections.EMBO molecular medicine · 2021Article
- Superoxide Dismutase Multigene Family from a Primitive Chondrostean Sturgeon,Antioxidants (Basel, Switzerland) · 2021Article
- The Role and Regulatory Network of the CiaRH Two-Component System in Streptococcal Species.Frontiers in microbiology · 2021Review
- An Inventory of CiaR-Dependent Small Regulatory RNAs inFrontiers in microbiology · 2021Review
- Von Willebrand Factor Mediates Pneumococcal Aggregation and Adhesion in Blood Flow.Frontiers in microbiology · 2019Article
- The VraSR regulatory system contributes to virulence in Streptococcus suis via resistance to innate immune defenses.Virulence · 2018Article
- Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis.BMC microbiology · 2016Article
- Infection of zebrafish embryos with live fluorescent Streptococcus pneumoniae as a real-time pneumococcal meningitis model.Journal of neuroinflammation · 2016Article
- Temporal Regulation of the Transformasome and Competence Development inFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus suis is an encapsulated Gram-positive bacterium, and the leading cause of sepsis and meningitis in young pigs resulting in considerable economic losses in the porcine industry. It is also considered an emerging zoonotic agent. In the environment, both avirulent and virulent strains occur in pigs, and virulent strains appear to cause disease in both humans and pigs. There is a need for a convenient, reliable and standardized animal model to assess S. suis virulence. A zebrafish (Danio rerio) larvae infection model has several advantages, including transparency of larvae, low cost, ease of use and exemption from ethical legislation up to 6 days post fertilization, but has not been previously established as a model for S. suis. Microinjection of different porcine strains of S. suis in zebrafish larvae resulted in highly reproducible dose- and strain-dependent larval death, strongly correlating with presence of the S. suis capsule and to the original virulence of the strain in pigs. Additionally we compared the virulence of the two-component system mutant of ciaRH, which is attenuated for virulence in both mice and pigs in vivo. Infection of larvae with the ΔciaRH strain resulted in significantly higher survival rate compared to infection with the S10 wild-type strain. Our data demonstrate that zebrafish larvae are a rapid and reliable model to assess the virulence of clinical porcine S. suis isolates.
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