ArticleF1000Research2016
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis .
Article in F1000Research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 13 citations in OpenAlex.
- Lysyl-Phosphatidylglycerol: A Lipid Involved in the Resistance ofAntibiotics (Basel, Switzerland) · 2025Review
- Structural insights into the transporting and catalyzing mechanism of DltB in LTA D-alanylation.Nature communications · 2024Article
- Pilot Lipidomics Study of Copepods: Investigation of Potential Lipid-Based Biomarkers for the Early Detection and Quantification of the Biological Effects of Climate Change on the Oceanic Food Chain.Life (Basel, Switzerland) · 2023Article
- Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum.Metabolites · 2023Article
- Design principles and functional basis of enantioselectivity of alanyl-tRNA synthetase and a chiral proofreader during protein biosynthesis.Nucleic acids research · 2023Article
- Exogenous fatty acids affect membrane properties and cold adaptation of Listeria monocytogenes.Scientific reports · 2022Article
- A partial reconstitution implicates DltD in catalyzing lipoteichoic acid d-alanylation.The Journal of biological chemistry · 2018Article
- Nutrient depletion-induced production of tri-acylated glycerophospholipids in Acinetobacter radioresistens.Scientific reports · 2018Article
- Comparative study on nutrient depletion-induced lipidome adaptations in Staphylococcus haemolyticus and Staphylococcus epidermidis.Scientific reports · 2018Article
- Deciphering the tRNA-dependent lipid aminoacylation systems in bacteria: Novel components and structural advances.RNA biology · 2018Review
- Characterization of N-Succinylation of L-Lysylphosphatidylglycerol in Bacillus subtilis Using Tandem Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis .F1000Research · 2016Article
- Alanylated lipoteichoic acid primer in Bacillus subtilis.F1000Research · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cationic modulation of the dominantly negative electrostatic structure of phospholipids plays an important role in bacterial response to changes in the environment. In addition to zwitterionic phosphatidylethanolamine, Gram-positive bacteria are also abundant in positively charged lysyl-phosphatidylglycerol. Increased amounts of both types of lipids render Gram-positive bacterial cells more resistant to cationic antibiotic peptides such as defensins. Lysyl and alanyl-phosphatidylglycerol as well as alanyl-cardiolipin have also been studied by mass spectroscopy. Phospholipids modified by other amino acids have been discovered by chemical analysis of the lipid lysate but have yet to be studied by mass spectroscopy. We exploited the high sensitivity of modern mass spectroscopy in searching for substructures in complex mixtures to establish a sensitive and thorough screen for aminoacylated phospholipids. The search for deprotonated aminoacyl anions in lipid extracted from Bacillus subtilis strain 168 yielded strong evidence as well as relative abundance of aminoacyl-phosphatidylglycerols, which serves as a crude measure of the specificity of aminoacyl-phosphatidylglycerol synthase MprF. No aminoacyl-cardiolipin was found. More importantly, the second most abundant species in this category is D-alanyl-phosphatidylglycerol, suggesting a possible role in the D-alanylation pathway of wall- and lipo-teichoic acids.
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