ArticleBiology of sex differences2016
Single-cell analysis reveals differential regulation of the alveolar macrophage actin cytoskeleton by surfactant proteins A1 and A2: implications of sex and aging.
Article in Biology of sex differences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 27 citations in OpenAlex.
- Surfactant proteins A and D nucleotide variants: association with retinal vascular disease.Pediatric research · 2026Article
- Deciphering the molecular landscape: evolutionary progression from gynecomastia to aggressive male breast cancer.Cellular oncology (Dordrecht, Netherlands) · 2024Article
- The alveolar macrophage toponome of female SP-A knockout mice differs from that of males before and after SP-A1 rescue.Scientific reports · 2022Article
- Neutrophil and Macrophage Response in Acinetobacter Baumannii Infection and Their Relationship to Lung Injury.Frontiers in cellular and infection microbiology · 2022Review
- The Lung Alveolar Cell (LAC) miRNome and Gene Expression Profile of the SP-A-KO Mice After Infection With and Without Rescue With Human Surfactant Protein-A2 (1AFrontiers in immunology · 2022Article
- Comparison of the Toponomes of Alveolar Macrophages From Wild Type and Surfactant Protein A Knockout Mice and Their Response to Infection.Frontiers in immunology · 2022Article
- Age-dependent increase of cytoskeletal components in sensory axons in human skin.Frontiers in cell and developmental biology · 2022Article
- How cell migration helps immune sentinels.Frontiers in cell and developmental biology · 2022Review
- Human Surfactant Protein SP-A1 and SP-A2 Variants Differentially Affect the Alveolar Microenvironment, Surfactant Structure, Regulation and Function of the Alveolar Macrophage, and Animal and Human Survival Under Various Conditions.Frontiers in immunology · 2021Review
- Surfactant Protein-A Function: Knowledge Gained From SP-A Knockout Mice.Frontiers in pediatrics · 2021Review
- Differences in the alveolar macrophage toponome in humanized SP-A1 and SP-A2 transgenic mice.JCI insight · 2020Article
- Differential Sex-Dependent Regulation of the Alveolar Macrophage miRNome of SP-A2 and co-ex (SP-A1/SP-A2) and Sex Differences Attenuation after 18 h of Ozone Exposure.Antioxidants (Basel, Switzerland) · 2020Article
- The Importance of Redox Status in the Frame of Lifestyle Approaches and the Genetics of the Lung Innate Immune Molecules, SP-A1 and SP-A2, on Differential Outcomes of COVID-19 Infection.Antioxidants (Basel, Switzerland) · 2020Review
- Impact of Surfactant Protein-A Variants on Survival in Aged Mice in Response toMicroorganisms · 2020Article
- Biological sex influences susceptibility to Acinetobacter baumannii pneumonia in mice.JCI insight · 2020Article
- Sex-Specific Regulation of Gene Expression Networks by Surfactant Protein A (SP-A) Variants in Alveolar Macrophages in Response toFrontiers in immunology · 2020Article
- Using toponomics to characterize phenotypic diversity in alveolar macrophages from male mice treated with exogenous SP-A1.Biomarker research · 2020Article
- Major Effect of Oxidative Stress on the Male, but Not Female, SP-A1 Type II Cell miRNome.Frontiers in immunology · 2019Article
- Differential Effects of Human SP-A1 and SP-A2 on the BAL Proteome and Signaling Pathways in Response toFrontiers in immunology · 2019Article
- Differential Impact of Co-expressed SP-A1/SP-A2 Protein on AM miRNome; Sex Differences.Frontiers in immunology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSurfactant protein A (SP-A) contributes to lung immunity by regulating inflammation and responses to microorganisms invading the lung. The huge genetic variability of SP-A in humans implies that this protein is highly important in tightly regulating the lung immune response. Proteomic studies have demonstrated that there are differential responses of the macrophages to SP-A1 and SP-A2 and that there are sex differences implicated in these responses.
methodsPurified SP-A variants were used for administration to alveolar macrophages from SP-A knockout (KO) mice for in vitro studies, and alveolar macrophages from humanized SP-A transgenic mice were isolated for ex vivo studies. The actin cytoskeleton was examined by fluorescence and confocal microscopy, and the macrophages were categorized according to the distribution of polymerized actin.
resultsIn accordance with previous data, we report that there are sex differences in the response of alveolar macrophages to SP-A1 and SP-A2. The cell size and F-actin content of the alveolar macrophages are sex- and age-dependent. Importantly, there are different subpopulations of cells with differential distribution of polymerized actin. In vitro, SP-A2 destabilizes actin in female, but not male, mice, and the same tendency is observed by SP-A1 in cells from male mice. Similarly, there are differences in the distribution of AM subpopulations isolated from SP-A transgenic mice depending on sex and age.
conclusionsThere are marked sex- and age-related differences in the alveolar macrophage phenotype as illustrated by F-actin staining between SP-A1 and SP-A2. Importantly, the phenotypic switch caused by the different SP-A variants is subtle, and pertains to the frequency of the observed subpopulations, demonstrating the need for single-cell analysis approaches. The differential responses of alveolar macrophages to SP-A1 and SP-A2 highlight the importance of genotype in immune regulation and the susceptibility to lung disease and the need for development of individualized treatment options.
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