Evidence map›Paper›PMID 26997482›Full record

ArticleCell2016

Programmable RNA Tracking in Live Cells with CRISPR/Cas9.

David A Nelles, Mark Y Fang, Mitchell R O'Connell, Jia L Xu, Sebastian J Markmiller, Jennifer A Doudna, Gene W Yeo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 262 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
262citing papers in PubMed, 2 pooled it
43.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

262 citing papers in PubMed, 2 syntheses or guidelines pooled it, 530 citations in OpenAlex.

  1. Engineering CRISPR guide RNAs for programmable RNA sensors.Biochemical Society transactions · 2023
    Pooled it
  2. Pooled it
  3. Article
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  6. Review
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  11. Bioluminescent Probes for Multiplexed RNA Imaging.Journal of the American Chemical Society · 2026
    Article
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  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
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  19. Review
  20. Article

202 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

David A NellesDepartment of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Materials Science and Engineering Graduate Program, University of California, San Diego, La Jolla, CA 92093, USA.
Mark Y FangDepartment of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92037, USA.
Mitchell R O'ConnellDepartment of Molecular and Cell Biology and Center for RNA Systems Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Jia L XuDepartment of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92037, USA.
Sebastian J MarkmillerDepartment of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92037, USA.
Jennifer A DoudnaDepartment of Molecular and Cell Biology and Center for RNA Systems Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, Innovative Genomics Initiative and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, Berkeley, CA 94720, USA.
Gene W YeoDepartment of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Materials Science and Engineering Graduate Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 1190777, Singapore. Electronic address: geneyeo@ucsd.edu.
University of California San Diego · USHoward Hughes Medical Institute · USNational University of Singapore · SGUniversity of California, Berkeley · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007198 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHI, NEIL C, INSEL, PAUL A · 1985 to 2024
$29.3M
Functional RNA elements in the human genomeR01HG004659 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett, Eugene Wei-Ming Yeo · 2008 to 2026
$12.4M
Defining the messenger RNP code in the brainR01NS075449 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI YEO, EUGENE WEI-MING · 2012 to 2016
$3.0M
Howard Hughes Medical InstituteNHGRI NIH HHS HG004659NHGRI NIH HHS R01 HG004659NIGMS NIH HHS T32 GM007198NINDS NIH HHS NS075449NINDS NIH HHS R01 NS075449
6 · The paper itself

Abstract

RNA-programmed genome editing using CRISPR/Cas9 from Streptococcus pyogenes has enabled rapid and accessible alteration of specific genomic loci in many organisms. A flexible means to target RNA would allow alteration and imaging of endogenous RNA transcripts analogous to CRISPR/Cas-based genomic tools, but most RNA targeting methods rely on incorporation of exogenous tags. Here, we demonstrate that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-programmed manner and allow endogenous RNA tracking in living cells. We show that nuclear-localized RNA-targeting Cas9 (RCas9) is exported to the cytoplasm only in the presence of sgRNAs targeting mRNA and observe accumulation of ACTB, CCNA2, and TFRC mRNAs in RNA granules that correlate with fluorescence in situ hybridization. We also demonstrate time-resolved measurements of ACTB mRNA trafficking to stress granules. Our results establish RCas9 as a means to track RNA in living cells in a programmable manner without genetically encoded tags.

Indexed as

CRISPR-Cas SystemsCytoplasmic GranulesEndonucleasesGlyceraldehyde-3-Phosphate DehydrogenasesGreen Fluorescent ProteinsHumansRNARNA, Guide, CRISPR-Cas SystemsRNA, MessengerEndonucleasesGlyceraldehyde-3-Phosphate DehydrogenasesGreen Fluorescent ProteinsRNARNA, Guide, CRISPR-Cas SystemsRNA, Messenger

Identifiers

PMID26997482
PMCPMC4826288
OpenAlexW2299638516

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.