ArticlePlant biotechnology journal2016
Metabolic engineering of folate and its precursors in Mexican common bean (Phaseolus vulgaris L.).
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Reconfiguring biofortification strategies to transform food systems and address micronutrient deficiency of the 21st century.Journal of integrative plant biology · 2026Review
- Dual-omics analysis reveals B vitamin regulation and metabolism in Areca Catechu inflorescence.BMC genomics · 2025Article
- Biological, dietetic and pharmacological properties of vitamin BNPJ science of food · 2025Review
- Strategies and bibliometric analysis of legumes biofortification to address malnutrition.Planta · 2024Review
- Biofortification of common bean (Food and energy security · 2023Review
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Nutritional assessment of nixtamalized maize tortillas produced from dry masa flour, landraces, and high yield hybrids and varieties.Frontiers in nutrition · 2023Article
- Reduced γ-glutamyl hydrolase activity likely contributes to high folate levels in Periyakulam-1 tomato.Horticulture research · 2023Article
- Effect of Amino Acids on Folates Accumulation in Wheat Seedlings during Germination under Red Light Radiation.Molecules (Basel, Switzerland) · 2022Article
- Applications of Genomic Tools in Plant Breeding: Crop Biofortification.International journal of molecular sciences · 2022Review
- Red light enhances folate accumulation in wheat seedlings.Journal of Zhejiang University. Science. B · 2021Article
- Molecular Mechanisms and Biochemical Pathways for Micronutrient Acquisition and Storage in Legumes to Support Biofortification for Nutritional Security.Frontiers in plant science · 2021Review
- Genome-Wide Association Study of Seed Folate Content in Common Bean.Frontiers in plant science · 2021Article
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.).PloS one · 2020Article
- Genetic Engineering for Global Food Security: Photosynthesis and Biofortification.Plants (Basel, Switzerland) · 2019Review
- Improved folate accumulation in genetically modified maize and wheat.Journal of experimental botany · 2019Article
- From Central to Specialized Metabolism: An Overview of Some Secondary Compounds Derived From the Primary Metabolism for Their Role in Conferring Nutritional and Organoleptic Characteristics to Fruit.Frontiers in plant science · 2019Review
- Toward Eradication of B-Vitamin Deficiencies: Considerations for Crop Biofortification.Frontiers in plant science · 2018Review
- Manipulation of Metabolic Pathways to Develop Vitamin-Enriched Crops for Human Health.Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Folate (vitamin B9) deficiency causes several health problems globally. However, folate biofortification of major staple crops is one alternative that can be used to improve vitamin intakes in populations at risk. We increased the folate levels in common bean by engineering the pteridine branch required for their biosynthesis. GTP cyclohydrolase I from Arabidopsis (AtGchI) was stably introduced into three common bean Pinto cultivars by particle bombardment. Seed-specific overexpression of AtGCHI caused significant increases of up to 150-fold in biosynthetic pteridines in the transformed lines. The pteridine boost enhanced folate levels in raw desiccated seeds by up to threefold (325 μg in a 100 g portion), which would represent 81% of the adult recommended daily allowance. Unexpectedly, the engineering also triggered a general increase in PABA levels, the other folate precursor. This was not observed in previous engineering studies and was probably caused by a feedforward mechanism that remains to be elucidated. Results from this work also show that common bean grains accumulate considerable amounts of oxidized pteridines that might represent products of folate degradation in desiccating seeds. Our study uncovers a probable different regulation of folate homoeostasis in these legume grains than that observed in other engineering works. Legumes are good sources of folates, and this work shows that they can be engineered to accumulate even greater amounts of folate that, when consumed, can improve folate status. Biofortification of common bean with folates and other micronutrients represents a promising strategy to improve the nutritional status of populations around the world.
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